FastQCFastQC Report
Fri 10 Feb 2017
SRR3557725.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557725.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1625076
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225841.3897196192670374No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106470.6551693582330919No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89180.5487743342465214No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39780.2447885514277486No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC35720.219805104499728No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT31180.19186794956051287No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC29010.1785147279265708No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA28050.17260731190418171No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA23730.14602393980343073No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA23010.1415933777866389No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT21850.13445525009291873No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT21160.13020929482682658No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19430.11956363886981285No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT17980.11064097925266264No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG2900.051.3108981
GACCGTT4150.042.976667
GTATCAA204950.042.429691
AACGCAG214750.041.0551386
TAACGCA2900.041.0007254
TATCAAC234650.036.9411052
ATCAACG235800.036.7345963
ACGCAGA243900.036.2458847
TCAACGC241200.036.158664
CGCAGAG245100.036.0199138
CAACGCA244750.035.6827745
TTAGGAC5200.035.441973
GCAGAGT256700.034.4616939
TAGGACC8500.032.1735084
ATAGTAC4950.031.2268124
GGACCGT7100.030.9815356
ATAACGC4700.030.3579833
ACATGGG188750.030.3318443
TACATGG199300.029.532542
GTACATG200600.029.3745941
Produced by FastQC (version 0.11.5)