FastQCFastQC Report
Fri 10 Feb 2017
SRR3557724.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557724.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3266212
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT347091.0626683142429212No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166640.5101934595794761No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130860.4006476003394759No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA66150.20252818861727284No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62880.19251659108471833No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT54620.167227356950498No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC39560.12111889858955881No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA34730.10633112608734521No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC33240.10176926666119653No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG5550.050.3930441
GTATCAA373200.034.9035721
AACGCAG389800.034.1306846
CATGGGT63400.034.13024
CATGGGG105900.030.7056354
ACGCAGA434750.030.6018187
CGCAGAG437850.030.3987318
ATCAACG433800.029.6410563
TATCAAC434900.029.6362462
TCAACGC442650.029.5721954
ACATGGG378300.029.4639953
CAACGCA446250.029.4135595
GCAGAGT459500.028.9664529
GTACATG413750.028.6063521
TACATGG412000.028.5851552
TAACGCA6450.028.5712154
ACGGTAC6950.027.3710773
GTATAGG8700.025.9914231
AGTACTT222050.025.35435312-13
ATGGGTA25550.024.8954285
Produced by FastQC (version 0.11.5)