FastQCFastQC Report
Fri 10 Feb 2017
SRR3557721.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557721.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1800864
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT204681.1365655596424826No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101510.5636738809815733No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83730.46494349378964767No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37530.20839996801535263No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA30650.17019608365762212No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA22860.12693906924676157No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT22100.12271887271887272No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG3550.056.991
TACAACG4050.041.106772
ACAACGC4300.038.7168433
AACGCAG219400.035.4742666
GTATCAA211700.035.0504571
TAACGCA5000.033.2964864
ATAGTAC6100.033.1405264
AAACGCA4750.032.5454375
ACGCAGA245700.031.628667
CGCAGAG247350.031.537868
ACATGGG207300.031.2061313
TATCACG2307.2759576E-1231.021572
CATGGGG55050.030.4580524
GTACATG220300.030.413891
ATCAACG244550.030.1727183
TAGTACT7100.030.1477225
TACATGG221600.030.1314742
TCAACGC249650.030.1041134
TATCAAC245250.030.08662
GCAGAGT260850.029.9740379
Produced by FastQC (version 0.11.5)