FastQCFastQC Report
Fri 10 Feb 2017
SRR3557719.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557719.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4161488
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT295860.7109476225811537No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129630.3114991560710976No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107050.2572397181008332No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47990.11531932808649215No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA47220.1134690283860004No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG6150.037.7427061
GTATCAA306050.037.007521
AACGCAG317350.036.5887766
ACGCAGA353250.032.786197
TCAACGC354900.032.1646924
ATCAACG349300.032.1186643
CGCAGAG360350.032.0907068
CAACGCA358550.031.8040945
TATCAAC355350.031.6391332
ACATGGG310850.031.271673
GTACATG333450.030.5217171
TACATGG336900.030.0364022
GCAGAGT386900.029.903939
TAACGCA7350.029.1205334
CATGGGG110900.028.3601344
CATGGGT43950.027.8671464
TATCACG5450.025.0911582
AGAGTAC374150.024.30544310-11
CATGGGC77700.024.2561784
AGTACTT210450.023.60577212-13
Produced by FastQC (version 0.11.5)