FastQCFastQC Report
Fri 10 Feb 2017
SRR3557718.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557718.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4353506
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT501601.1521748218562233No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT238210.5471681904193999No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181110.4160095334656711No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90620.20815407168383368No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA80960.18596505896626767No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT73090.1678876748992651No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC62810.14427452264910168No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC54560.12532427886857167No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA49870.11455135240424615No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46570.10697125489203414No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCAG541050.037.2720266
GTATCAA533650.037.1614071
CGCATAG7500.036.503981
ACGCAGA606000.033.130127
CGCAGAG608750.033.048828
TATCAAC616600.031.9738942
ATCAACG623200.031.5193423
GCAGAGT640350.031.4643489
TCAACGC631850.031.4642144
CAACGCA638450.031.1948225
CATGGGT68300.030.5527974
CATGGGG142550.029.3609454
ACATGGG501350.028.0923843
GACCGTT7400.027.3156627
GTACATG548250.026.792311
TACATGG548650.026.766162
TACAACG7800.026.678292
ACGGTAC9950.026.2902163
GTACTGG30550.026.1058141
CATGGGC108000.025.8174694