FastQCFastQC Report
Fri 10 Feb 2017
SRR3557717.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557717.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5469086
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT464090.8485695781708315No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT204910.37466955173131306No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159810.2922060468604809No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78200.1429855006851236No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA76990.1407730651885891No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT67690.12376839566977005No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC56390.10310680797486088No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA467850.038.4586941
AACGCAG508350.036.324956
ACGCAGA567050.032.501757
ATCAACG551600.032.4097443
TATCAAC553400.032.3908652
TCAACGC560600.032.3560524
CGCAGAG571300.032.1767128
CAACGCA568150.032.0307245
CATGGGT71400.030.9749874
CATGGGG164600.030.6289754
GCAGAGT609700.030.2086739
ACATGGG499600.030.178193
GTACATG531850.029.5781521
TACATGG533700.029.4647562
GTACTGG39000.026.0972351
CGCATAG7850.025.779371
GGACCGT14250.025.4496256
TAACGCA9800.024.8727744
AGTACTT309900.024.54871712-13
AGAGTAC593200.024.54049310-11
Produced by FastQC (version 0.11.5)