FastQCFastQC Report
Fri 10 Feb 2017
SRR3557716.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557716.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5390969
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT711351.3195215925003465No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT345310.6405341970988889No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT253280.4698227721213014No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA142140.2636631744682635No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT129160.23958587036950127No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128630.23860274470137002No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA70010.1298653358978692No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63890.11851301686208918No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG57310.10630741894453483No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC56080.10402582541283395No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG55770.10345078964468168No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG8300.041.5863651
GTATCAA745150.035.5399971
AACGCAG763750.035.2121776
TAACGCA9100.032.0092964
CATGGGG178900.031.7664744
ACGCAGA849350.031.5933957
CGCAGAG853950.031.485868
CATGGGT82800.031.4459884
TATCACG8900.031.3933282
TATCAAC857700.030.731932
ATCAACG864600.030.4035973
TCAACGC879600.030.148694
GCAGAGT894200.030.0553179
CAACGCA888950.029.9385835
ACATGGG716500.027.860313
GTACATG750250.027.3820421
TACATGG751700.027.283752
ATGGGTG50000.026.869525
TACAACG10100.026.4862612
AGTACTT436650.026.23504412-13