FastQCFastQC Report
Fri 10 Feb 2017
SRR3557714.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557714.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6370009
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT715961.1239544559513182No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT347970.5462629644636295No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT262780.41252688967943374No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133790.21003110042701667No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA71440.11215054798195732No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66720.10474082532693439No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCAG666300.042.2674376
GTATCAA653600.042.1793141
CGCATAG10450.042.1462021
ACGCAGA755250.037.226397
CGCAGAG761350.037.029648
TATCAAC763800.035.9779362
ATCAACG770250.035.5906373
TCAACGC786450.035.394244
GCAGAGT808300.034.9008459
CAACGCA798500.034.8898965
TATCACG10000.032.700072
GTACATG651800.031.5026991
ACATGGG633200.031.4821933
TACATGG657350.031.2490882
TACAACG12550.030.7932572
CATGGGG198450.030.3480434
CATGGGT85650.030.333774
AGAGTAC794500.028.29406510-11
ACAACGC14100.028.2506223
TAACGCA11000.027.0240024
Produced by FastQC (version 0.11.5)