FastQCFastQC Report
Fri 10 Feb 2017
SRR3557712.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557712.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3325346
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT427171.2845881300772912No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT208080.6257393967424744No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160710.48328805483700044No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82030.24668109724521897No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA75550.22719440322901738No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT62570.18816087107928017No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC55600.16720064618839664No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA52500.15787830800163352No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC52430.1576678035909647No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA40760.12257371112660156No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39970.12019801849191031No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA39740.11950636114256982No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT39120.1176418935052172No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG36540.10988330236913692No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG7050.044.7432061
AACGCAG481750.036.1943366
GTATCAA474350.035.6085131
TACAACG6950.035.072152
ACGCAGA536650.032.3919077
CGCAGAG538200.032.386998
ACAACGC7900.032.3587463
CATGGGG113700.031.6856384
TAACGCA6250.031.3894864
TATCAAC541000.031.0115222
GCAGAGT563600.031.0012289
TATCACG6950.030.7950572
ATCAACG547900.030.6309093
TCAACGC560700.030.4829924
GTATAAC8250.030.2995171
CAACGCA568050.030.1932265
ACATGGG441650.029.9503613
GTACATG473600.029.230641
AGATTAC13450.029.1732622
TCACGCA7550.029.1343334
Produced by FastQC (version 0.11.5)