FastQCFastQC Report
Fri 10 Feb 2017
SRR3557711.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557711.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3465886
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT430271.2414430249581203No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197270.5691762510365315No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157640.45483319416737883No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75170.2168853793806259No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA48960.14126258047725748No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA46590.1344245021330765No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT41850.12074834544471458No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39140.11292927695833042No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC38590.11134238114006059No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC37500.10819744215476215No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA36550.10545644028684151No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCAG407800.039.2745256
GTATCAA409550.038.6790121
CGCATAG6900.036.222171
TACAACG5600.036.0953642
ACGCAGA458700.034.8515937
CGCAGAG462450.034.568988
TATCAAC474050.033.2966772
ATCAACG475300.033.1090473
GCAGAGT486750.032.8554089
TCAACGC485850.032.720484
CAACGCA490450.032.522695
ACATGGG383250.031.4280763
CATGGGG108800.031.3648724
GTACATG404450.030.897881
TACATGG406450.030.8628372
CATGGGT40450.027.4842324
CATGGGC98600.027.0725984
AGAGTAC477550.027.02878810-11
ACAACGC7700.026.2511733
AGTACTT269500.025.50426712-13
Produced by FastQC (version 0.11.5)