FastQCFastQC Report
Fri 10 Feb 2017
SRR3557710.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557710.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3614342
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT515101.4251556714887523No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT248990.6888944101028625No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188730.5221697337994025No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93390.25838728045104753No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA85850.23752594524812537No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT68600.18979941577194412No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC53510.1480490778127803No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA52860.14625068684701115No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC50820.14060650596982796No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48690.13471331711276907No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG42340.11714442075487046No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT41290.11423932765632029No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA37730.10438967867456926No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC37710.1043343435679302No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA36290.10040555099655761No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG7850.047.015441
TACAACG5250.045.3004152
AACGCAG532900.036.4724276
GTATCAA536000.035.427821
TAGTACT11300.033.674185
ACGCAGA591750.032.7748837
CGCAGAG594350.032.7015348
ACAACGC7700.032.430533
TAACGCA8500.032.1761744
CATGGGG117600.032.0536424
GCAGAGT621800.031.2196459
CATGGGT51450.031.2013874
TATCACG6700.031.0595192
ATAGTAC12100.030.9564174
TATCAAC611600.030.894982
ATCAACG613400.030.7651213
AGATTAC15400.030.5005652
TCAACGC627700.030.4620694
CAACGCA633900.030.2297845
ACATGGG471350.029.7689843
Produced by FastQC (version 0.11.5)