FastQCFastQC Report
Fri 10 Feb 2017
SRR3557709.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557709.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1700010
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240381.4139916824018681No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113640.6684666560784936No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90340.531408638772713No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41820.24599855294968853No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA25990.152881453638508No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA25070.14746972076634843No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23640.13905800554114386No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC22030.12958747301486462No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT21740.12788160069646648No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC21420.12599925882788926No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19670.11570520173410745No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT18090.1064111387580073No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17170.10099940588584773No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG3750.052.344331
GTATCAA221300.039.2964131
AACGCAG228850.038.877656
GGACCGT4650.035.787746
ACGCAGA254700.034.8385477
CGCAGAG256300.034.667448
TATCAAC255100.033.8752982
ATCAACG256250.033.5841063
TCAACGC262200.033.3206754
CATGGGT22200.033.1969264
CAACGCA263600.033.1437075
GCAGAGT268400.033.038139
ACATGGG215700.031.4387053
GACCGTT3800.031.2806367
GTACATG223350.031.159241
TACATGG224350.030.9948062
CATGGGG62200.030.7676724
TAACGCA3350.030.1601354
ATGGGTG14500.029.5116625
ATAGTAC6650.029.493174