FastQCFastQC Report
Fri 10 Feb 2017
SRR3557708.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557708.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6689901
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT843741.261214478360741No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT417550.6241497445178935No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT288130.43069396692118467No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149660.2237103359227588No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA79870.11938891173426931No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75740.11321542725370673No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75060.11219896976053907No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA660000.044.819221
AACGCAG685150.043.913166
ACGCAGA775650.038.7282337
CGCAGAG779000.038.6150938
TATCAAC779550.037.811272
ATCAACG783650.037.6126063
TCAACGC796750.037.3448074
CGCATAG11100.036.9830931
CAACGCA805250.036.9355665
GCAGAGT830800.036.2718479
ACATGGG647650.033.066283
GTACATG664150.032.9206431
TACATGG673050.032.4549672
CATGGGG204150.030.017244
AGAGTAC810850.029.43454410-11
CATGGGT90850.028.917374
AGTACTT489000.027.3909412-13
CATGGGC168450.027.09894
TATCACG10750.027.0931072
CATGGGA322150.025.7935144
Produced by FastQC (version 0.11.5)