FastQCFastQC Report
Fri 10 Feb 2017
SRR3557707.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557707.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3051386
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT347331.138269625671744No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173170.5675125991926292No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124330.40745418639267533No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63990.20970798188102063No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA47320.15507706989545078No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38510.12620494424500867No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA35830.11742205017654272No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT32630.10693501248285205No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC31980.10480483295132115No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG6300.053.825291
ACAACGC5950.037.956973
TAACGCA6950.035.9161154
AACGCAG359350.035.657926
GTATCAA351250.035.0764661
TACAACG6650.033.9637222
AAACGCA8050.033.2232065
CATGGGG96400.032.367354
ACGCAGA397300.032.266847
CGCAGAG399800.032.1839948
ACATGGG341250.031.8018933
TACATGG361800.030.8682172
GTACATG364050.030.6892761
TATCAAC400850.030.6338632
ATCAACG400050.030.6336883
GCAGAGT421200.030.5205979
TCAACGC410050.030.4808654
CAACGCA415050.030.1852725
CATGGGC79600.030.1642954
CATGGGT37500.030.1125284