FastQCFastQC Report
Fri 10 Feb 2017
SRR3557706.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557706.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3790493
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT334950.8836581415662818No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167140.44094528073261186No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126050.3325424951318997No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59630.15731462899417042No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA52740.13913757392508044No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT40510.10687264163263197No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA325400.039.7866251
CGCATAG6150.039.6647261
AACGCAG356350.037.309616
TAACGCA6150.035.7567564
ATCAACG383550.033.8584183
TATCAAC383950.033.701532
TCAACGC389550.033.626364
CAACGCA392200.033.414315
ACGCAGA397300.033.3892867
CGCAGAG401800.033.015348
GCAGAGT426800.031.0953739
ACATGGG359000.030.3793743
GTACATG381250.029.7758581
TACATGG381750.029.6295872
CATGGGG121850.028.9241524
GTATAGG10200.026.2487181
CATGGGT49650.026.2153914
AGAGTAC415000.025.46399910-11
AGTACTT216300.024.55166812-13
CATGGGC85600.024.2316594
Produced by FastQC (version 0.11.5)