FastQCFastQC Report
Fri 10 Feb 2017
SRR3557705.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557705.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1912215
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT226761.1858499175040462No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113690.5945461153688262No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88390.4622388172878049No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42780.2237196131188177No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25510.13340550095046844No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA24280.1269731698579919No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA24190.12650251148537167No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20270.10600272458902373No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG3450.044.840531
TATCACG3300.041.4306142
TACAACG3800.040.672162
TAACGCA3150.039.6292844
AACGCAG220400.038.1099246
ACAACGC3950.037.6227383
GTATCAA220450.037.003591
AGATTAC8950.035.865612
CATGGGT29900.035.7856454
CATGGGG66250.035.4420364
ACATGGG219300.034.7501643
GTACATG229850.034.2477341
TACATGG230800.033.997392
ACGCAGA246800.033.985167
CGCAGAG247850.033.8891538
TCACGCA4000.032.694164
GCAGAGT260900.032.148489
ATCAACG254550.031.9696463
TATCAAC254500.031.9292162
TCAACGC258400.031.90744