FastQCFastQC Report
Fri 10 Feb 2017
SRR3557704.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557704.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4450087
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT571541.284334441101938No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT288410.6480996888375441No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT211750.4758333938190422No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105440.23693918793048313No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA71630.16096314521491378No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55830.12545822137859328No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC55590.12491890607981374No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC54830.12321107430034513No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT53080.1192785669134109No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA51450.11561571717586645No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT49950.11224499655849425No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49580.11141355213954245No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44650.10033511704377915No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG7250.043.487151
GTATCAA516950.040.252641
AACGCAG542650.038.9939236
ACGCAGA613350.034.3440937
CGCAGAG616600.034.230558
TATCAAC605450.034.1937562
ATCAACG606150.034.1931083
TCAACGC618150.033.9139484
CAACGCA623350.033.659645
ACGGTAC9500.033.1603433
GCAGAGT649200.032.5665789
ACATGGG519500.032.036073
GTACATG554850.031.1561031
TACATGG554750.031.0936532
CATGGGT63050.031.0154114
GGACCGT13100.029.946046
CATGGGG141300.029.7822724
CATGGGC115700.029.0770154
TATCACG8000.028.9765222
TACAACG6850.028.6348552