FastQCFastQC Report
Fri 10 Feb 2017
SRR3557702.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557702.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2349295
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT377541.6070353020799857No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180350.7676771116441315No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134990.5745979112882801No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67430.2870222768958347No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA42350.18026684601125018No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA39520.16822067896964835No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33960.1445540044992221No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33730.14357498738983399No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC30890.13148625438695438No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT29720.1265060369174582No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC29530.12569728365318106No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26420.11245926969580236No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA26220.11160795047024745No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT25520.1086283331808053No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA25520.1086283331808053No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA25330.10781957991652816No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25030.1065426010781958No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG4650.047.3360631
AACGCAG344350.039.9581956
GTATCAA350050.038.951831
TACAACG4800.037.148752
CGCAGAG382400.036.091038
ACGCAGA381950.036.024627
TAACGCA5850.034.5444264
GCAGAGT401100.034.4528589
CATGGGG83050.033.9945224
GTATAAC7000.033.9942361
TATCAAC398000.033.990172
ATCAACG400550.033.847263
TCAACGC408750.033.546314
CAACGCA414450.033.027585
ACATGGG316050.032.703843
GTACATG330700.032.6322021
TACATGG331500.032.489192
ATGGGAC27550.032.145465
ACAACGC5850.031.496393
CATGGGT28800.029.0992364
Produced by FastQC (version 0.11.5)