FastQCFastQC Report
Fri 10 Feb 2017
SRR3557700.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557700.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences690423
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80951.172469630936397No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42010.6084675626391357No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33550.4859339853973578No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA17630.25535070529226284No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17190.24897780056574015No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13770.1994429501914044No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT11090.1606261668571296No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA10380.15034261604842247No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA8980.13006519191857746No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8140.11789873744067042No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG7760.11239486517685535No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT7540.10920841281359399No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC7290.10558744421897881No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7040.1019664756243636No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA6950.10066292693030215No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCA2050.052.2005354
CGCATAG1400.051.0167771
GTATAAC2550.044.347921
TATACCG1109.6933945E-943.2368055
AGATTAC3250.042.075792
GTATCAC2200.037.8760951
ATAACGC3150.037.7464183
TACAACG957.5068638E-637.5504722
TATCACG1801.4551915E-1136.333562
ATCACGC1801.4551915E-1136.330933
TCACGCA2500.035.670374
GATTACT3850.035.5159453
ACGCATA851.7064724E-435.0115171
GGTTTCA3950.034.6569671
GTACTGG4300.033.220231
CATGGGG27200.033.0038344
CATGGGC22800.031.550544
ACATGGG104550.031.0473773
ATGGGAC8250.030.986385
ACAACGC1352.343897E-630.826243
Produced by FastQC (version 0.11.5)