FastQCFastQC Report
Fri 10 Feb 2017
SRR3557699.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557699.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1785918
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290821.6284062314171197No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140310.7856463734617155No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105650.5915725134076705No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55090.3084688098781691No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA34500.1931779622580656No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA33690.1886424796659197No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30680.1717884023790566No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27310.15291855505123975No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT24500.13718435000935092No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23570.13197694407022048No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA21790.12201008108994925No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21610.1210021960694724No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA20780.11635472625282908No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA20270.11349905202814464No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC18160.10168439984366584No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG4900.070.411461
ATAGTAC7550.047.232254
GCATAGT7700.044.7684022
TAGTACT8500.043.3517955
AGATTAC7100.040.1806762
TATCACG3000.039.6226122
CATGGGT32650.039.501254
AACGCAG271450.037.7250446
TCACGCA3550.036.8322834
GTATCAA279100.035.8703581
ACATGGG256400.034.3993453
CATGGGG63100.034.1909834
TACATGG268950.033.700212
GTACATG268100.033.681111
CGCAGAG305200.033.6701288
ACAACGC3900.033.526823
ACGCAGA305750.033.512377
ATGGGTG19900.032.8529175
GCAGAGT319550.032.1953059
CATGGGC59050.031.7047944
Produced by FastQC (version 0.11.5)