FastQCFastQC Report
Fri 10 Feb 2017
SRR3557698.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557698.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5258534
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT675681.284920854367396No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT346710.6593282462374495No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT238370.45330124327426613No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123990.23578814932070422No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA114110.2169996428662437No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC99040.18834146551111014No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC91000.17305203313318884No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT89410.17002837673009247No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT88510.1683168731056983No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA66100.1257004328582833No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA64900.12341842802575775No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64260.12220135878174411No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT62650.11913966896477232No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60500.115051076973164No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT57650.10963131549591577No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG10150.043.371531
GTATCAA664250.035.760761
AACGCAG693450.035.0194326
TAACGCA9050.032.835784
CGCAGAG770500.031.671768
ACGCAGA769250.031.6073367
GTACTGG43250.031.4983921
ACATGGG609450.031.0599983
TATCAAC764200.030.9307882
CATGGGG172950.030.858994
ATCAACG774650.030.6200713
TCAACGC790250.030.4064084
CATGGGC140200.030.309864
CAACGCA792900.030.3047875
TACTGGT40650.030.2658042
GCAGAGT808350.030.1667129
CATGGGT56150.030.166234
TACAACG9050.029.5533282
TACATGG675350.029.4996952
GTACATG676950.029.4920121
Produced by FastQC (version 0.11.5)