FastQCFastQC Report
Fri 10 Feb 2017
SRR3557697.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557697.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2957913
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT464971.5719529276216035No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225050.7608404980132952No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164590.5564396248300745No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81890.2768506037871972No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA54510.1842853390211274No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42730.14445996214222662No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39980.13516286652109105No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34490.11660248289926038No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA31230.10558119863565966No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA30160.10196378324852692No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29800.10074670891266917No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCAG361800.044.4229746
GTATCAA360100.043.4571271
CGCATAG6150.039.652631
ACGCAGA411750.039.0772677
CGCAGAG414400.038.9134378
GCAGAGT433800.037.26919
TATCAAC420300.037.2364582
ATCAACG423050.037.1342773
TCAACGC429900.036.9711954
CAACGCA435600.036.4464725
GTACATG338850.033.3510441
TAACGCA5900.033.245394
ACATGGG331850.033.1538623
TACATGG339150.033.0892562
TACAACG5800.030.7446922
CATGGGG98250.030.5512074
CATGGGC80600.030.0143474
AGAGTAC428600.030.00366210-11
TATCACG4450.029.3859392
AGATTAC11950.028.3520072