FastQCFastQC Report
Fri 10 Feb 2017
SRR3557694.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557694.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1721503
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137910.801102292589673No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67180.39024038877655165No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51640.2999704328136518No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA26510.15399334186463803No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25870.1502756602805804No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT17960.10432743945261785No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17740.10304948640809805No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG2750.038.942581
TAACGCA2900.034.8392564
AACGCAG162650.033.76266
GTATCAA159300.033.5759931
CATGGGG49750.030.8208474
ACGCAGA180100.030.6233167
ACATGGG161750.030.5342523
CGCAGAG182150.030.3439228
GTACATG173650.029.9790731
TACAACG3400.029.71672
TACATGG173600.029.6482282
CATGGGC41200.029.4273324
TCAACGC183800.029.1984774
ATCAACG181150.029.1015323
GTATAAC5350.028.9137541
CAACGCA185650.028.8434895
GCAGAGT192700.028.5592779
TATCAAC184750.028.5022962
ACAACGC4250.027.968663
CATGGGT18750.025.9914514
Produced by FastQC (version 0.11.5)