FastQCFastQC Report
Fri 10 Feb 2017
SRR3557692.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557692.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1990353
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191910.9642008226681398No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96180.4832308640728554No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72610.36480965939207766No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35490.1783100786644379No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA32570.16363931423219902No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25480.12801749237446824No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT21540.10822200885973493No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG4550.048.3727571
TATCACG3450.046.5152132
TAACGCA4000.041.605274
AACGCAG217000.035.1960336
GTATCAA206100.035.0100981
ATAACGC6600.033.320243
GTATAAC5900.033.2715031
TCACGCA5200.033.147064
ACATGGG206050.032.710773
GTACATG223100.031.8624271
CATGGGG68300.031.8500964
TACATGG223500.031.5131032
ACGCAGA242200.031.484947
CGCAGAG243650.031.4195378
ATCAACG237200.030.4447173
TATCAAC238300.030.3291242
TCAACGC241700.030.3205284
CAACGCA245100.030.215175
CATGGGC48900.030.0219174
ATCACGC5550.029.985783
Produced by FastQC (version 0.11.5)