FastQCFastQC Report
Fri 10 Feb 2017
SRR3557690.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557690.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2207452
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT273341.2382602203807829No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131970.597838593998873No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101200.45844711459184617No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48000.21744527174316813No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA29490.1335929388272089No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA27020.12240356755209172No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25320.11470238084452118No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23920.10836022708534546No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA245900.039.980551
AACGCAG259000.038.711926
CGCATAG4250.036.3847051
ACGCAGA289200.034.731047
CGCAGAG291650.034.4596638
TACAACG4150.034.371032
TATCAAC286700.034.14252
ATCAACG289700.033.624813
ACAACGC3900.033.5264433
TCAACGC295050.033.4582634
CAACGCA297150.033.1418045
GCAGAGT308050.032.6443869
ACATGGG247250.031.7778533
GTACATG261150.031.337391
CATGGGT30750.031.3111674
TATCACG4400.031.0673752
TACATGG262950.030.9880082
GTATAAC6300.029.2654761
CATGGGG76850.029.155934
TAACGCA4900.029.1101574