FastQCFastQC Report
Fri 10 Feb 2017
SRR3557688.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557688.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3542769
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT283760.800955410866472No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136790.38611041250502076No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99050.27958356867184964No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52180.14728592239573057No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA42780.1207530042178872No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA291500.037.6930581
AACGCAG303550.037.0806546
CGCATAG5550.036.443411
ACGCAGA337950.033.2358677
CGCAGAG343150.032.8361328
ATCAACG336750.032.3130843
TATCAAC336800.032.3096542
GTACATG328850.032.1456831
ACATGGG316200.032.1389663
TCAACGC343100.032.113434
CAACGCA347800.031.7819845
TACATGG333050.031.7455272
TAACGCA6400.031.5716024
GCAGAGT367750.030.7042569
CATGGGG100250.030.6481194
CATGGGT48250.029.314154
TATCACG5450.026.171632
AGAGTAC355800.025.57213210-11
AGTACTT187100.024.79115512-13
GTATAAC10800.024.7868771
Produced by FastQC (version 0.11.5)