FastQCFastQC Report
Fri 10 Feb 2017
SRR3557687.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557687.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3369258
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT398971.1841479637356356No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207700.6164562048973394No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147470.43769280951473594No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72820.21613067328177302No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA47110.1398230708363681No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA42880.12726837778525718No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40560.12038258868866676No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37500.11130047031126734No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT37400.11100366905710396No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG6750.043.1952131
GTATCAA379200.039.3238531
AACGCAG389750.038.8415076
TAACGCA6800.037.5854034
ACGCAGA434250.034.8748867
CGCAGAG436650.034.7104268
ACATGGG380600.033.576033
ATCAACG444150.033.388783
TATCAAC444800.033.3409772
CATGGGT60550.033.277084
GTACATG395200.033.169651
TCAACGC452150.033.1003724
GCAGAGT460700.032.9371349
TACATGG396450.032.9242972
CAACGCA457750.032.6949425
CATGGGG104250.032.384114
ATGGGTG37650.029.2051855
TATCACG4900.029.1130492
CATGGGC89950.029.0083484
GTACTGG22800.028.9688631