FastQCFastQC Report
Fri 10 Feb 2017
SRR3557685.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557685.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3272801
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT484551.4805360912563885No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT235160.7185282575995302No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168980.5163161463223703No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84420.2579441890906291No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA49880.15240767770481617No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43510.13294422728421312No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43420.13266923347921245No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35960.10987530253137909No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33860.10345878041469678No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA375600.045.070551
AACGCAG392750.043.9025466
ACGCAGA440550.039.1525847
CGCAGAG443400.038.9277348
TATCAAC448000.037.7735252
ATCAACG451100.037.591283
TCAACGC461300.037.1208574
GCAGAGT469250.036.7832839
CAACGCA465950.036.699385
GTACATG370200.032.355161
ACATGGG364800.032.2277263
TACATGG371950.032.105062
CATGGGT48000.032.0713124
CGCATAG5150.031.1955531
CATGGGG115050.030.583934
AGAGTAC460250.029.8767910-11
TAACGCA5500.028.0975764
AGTACTT268000.027.3233612-13
GGTATCA227200.027.158551
GGACCGT8450.026.025756