FastQCFastQC Report
Fri 10 Feb 2017
SRR3557683.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557683.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3094507
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT398071.286376149738876No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196530.6350930859099688No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145960.4716744864367733No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70750.22863092570157378No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA43480.14050703391525693No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39290.12696691266169377No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36680.11853261278775586No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA33990.10983979031231791No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31830.10285968007181757No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCAG350600.041.774336
GTATCAA348600.041.3640331
CGCATAG5900.038.3132671
ACGCAGA393750.037.15117
CGCAGAG393150.037.1322068
TATCAAC405650.035.4165152
ATCAACG407300.035.2876363
TCAACGC415550.035.0590974
GCAGAGT417800.035.041019
CAACGCA419350.034.599665
GTACATG333150.030.9261381
CATGGGT39350.030.8152794
ACATGGG326750.030.7979643
TACATGG337500.030.5566942
TAACGCA5100.030.3028764
CATGGGG95050.030.079714
AGAGTAC408600.028.49815410-11
AGTACTT238200.026.82547412-13
GAGTACT216400.025.28408212-13
CATGGGC85700.024.1367664
Produced by FastQC (version 0.11.5)