FastQCFastQC Report
Fri 10 Feb 2017
SRR3557682.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557682.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1223243
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120410.9843506155359156No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60010.4905811846051847No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48710.39820379107013076No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA38250.31269338962086846No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT26670.21802699872388395No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24560.20077776860362168No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16550.13529609407125157No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT15910.13006410010112462No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC14880.12164385980545156No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG13900.11363236903869468No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG13140.10741937619916893No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA12630.10325013100422403No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCA2850.041.7021684
GCGTAAT600.001310809439.6527181
GTATAAC3500.037.3868451
CATGGGG40700.036.356194
CATGGGT19300.032.022084
CGCATAG2650.031.4229071
GTACTGG7750.030.6988771
ATAACGC5650.030.5016293
TACTGGT6850.030.3634362
ACATGGG158150.030.3233953
GTACATG174450.029.3900591
TACATGG175400.029.238472
TACAACG2252.0918378E-1029.0525072
GGACCGT3300.028.8124056
TATCACG2455.620677E-1026.6808762
ACGCATA1358.2663646E-526.4351431
AACGCAG163200.026.2535846
CATGGGC29300.025.7578474
ACGGTAC2558.949428E-1025.6345673
TCACGCA2809.640644E-1125.4681074
Produced by FastQC (version 0.11.5)