FastQCFastQC Report
Fri 10 Feb 2017
SRR3557681.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557681.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1756257
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164940.9391563990919325No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84290.47994114756553286No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66010.3758561531712044No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33690.1918284169116479No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21350.12156535176799295No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA20000.11387854966556717No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA19000.10818462218228882No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG3750.039.6617741
AACGCAG161950.037.0288856
GTATCAA158600.036.7609141
ACATGGG162400.034.547483
TACATGG169350.033.8666732
GTACATG170550.033.696811
CATGGGG53400.033.6121374
ACGCAGA180600.033.205037
CGCAGAG183600.032.6624648
ATCAACG179650.032.1896363
TCAACGC184050.031.9690574
TATCAAC181500.031.9597682
CAACGCA186800.031.5302355
GCAGAGT194700.030.7698239
ACGCATA1951.4242687E-930.5090581
TAACGCA2850.029.1953454
CATGGGT18700.027.9686494
CATGGGC40800.027.385974
TACAACG3700.027.3072282
GTGTTAT7300.026.8939441
Produced by FastQC (version 0.11.5)