FastQCFastQC Report
Fri 10 Feb 2017
SRR3557680.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557680.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences750188
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89031.1867691831914133No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44120.5881192447759762No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35140.4684159170767861No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17690.23580755757223523No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA15330.20434877657333894No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14500.19328488325593052No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA10610.1414312145755464No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA10320.1375655169104278No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT9590.12783462278788785No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8760.1167707294704794No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8160.10877273430126848No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAACG1400.063.6774062
CGCATAG1500.059.4758531
ACAACGC1850.048.188313
TAACGCA1400.046.6967664
ACGGTAC1256.2937033E-1042.7912183
GTACAAC2350.040.4941981
ATAGTAC2450.036.387094
AACGCAG95150.035.7280586
CACGGTA1503.754394E-935.6593482
GGACCGT1703.2923708E-1034.9601446
TAGTACT2950.034.2490925
GTATCAA98850.033.453291
ACATGGG94650.032.651633
GTACATG98600.032.6333051
CATGGGG23750.032.5313344
TACATGG98500.032.4614682
TATCACG1301.7505135E-632.001982
TTCAACG3900.032.001984
ACGCAGA107000.031.8823497
GTATAAC2255.456968E-1231.7204531
Produced by FastQC (version 0.11.5)