FastQCFastQC Report
Fri 10 Feb 2017
SRR3557677.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557677.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2094199
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT243591.1631654871385193No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117200.5596411802316781No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87410.4173910884304691No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44770.21378102081034325No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA29240.1396237893342514No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA26400.1260625184139616No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22620.10801265782287164No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT21650.10338081529023746No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21420.10228254334950977No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG3750.055.5391461
TAACGCA3950.040.6324124
AACGCAG236750.039.4701236
GTATCAA230900.039.0437131
ACGCAGA268800.034.786077
CGCAGAG270450.034.6397828
TATCAAC267500.033.579772
ATCAACG269950.033.4267653
TCAACGC275700.033.1608354
CAACGCA278450.032.8546835
GCAGAGT287750.032.515869
TACAACG3700.032.1340372
ACATGGG226300.031.5999933
CATGGGG69050.031.422094
GTACATG237450.031.2504791
TACATGG239350.031.0217322
CATGGGT27800.029.9356844
ATAGTAC7600.028.1575474
TAGTACT8100.027.1533015
AAACGCA7300.026.8718155