FastQCFastQC Report
Fri 10 Feb 2017
SRR3557673.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557673.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4530002
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT573641.2663129067051184No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT269860.5957171762838074No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199270.43988943051239277No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99400.21942595168832155No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA60500.1335540249209603No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52620.11615888911307325No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52190.11520966215908955No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT47900.10573946766469419No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA45910.10134653362183946No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA520350.040.1277121
AACGCAG545450.038.854566
CGCATAG7450.035.9326361
ACGCAGA604200.035.0666667
CGCAGAG607700.034.9233788
TATCAAC609900.034.1748922
ATCAACG614500.033.9183123
TCAACGC623750.033.6436234
CAACGCA630550.033.3373575
GCAGAGT640850.033.1910449
CATGGGG152350.030.895724
ACATGGG501900.029.4732573
GTACATG539050.028.63791
CATGGGT65200.028.5306934
TACATGG541650.028.4301172
AGTACTT327950.027.11092812-13
AGAGTAC627300.026.91162310-11
GAGTACT294200.025.8676712-13
CATGGGC115450.025.224224
TAACGCA8300.024.3453755
Produced by FastQC (version 0.11.5)