FastQCFastQC Report
Fri 10 Feb 2017
SRR3557670.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557670.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2391713
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270421.1306540542280783No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136850.5721840371315454No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96090.40176225157449913No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50480.2110621132217787No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA41990.17556454307017605No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT32190.1345897271119068No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29900.1250149997094133No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA27950.11686184755445156No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC27390.11452042949969331No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC24070.10063916531791231No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24040.10051373220783598No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT24010.10038829909775965No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG4500.042.300981
AACGCAG271850.035.699416
GTATCAA266700.035.686991
TAACGCA4350.034.1557664
CATGGGG72350.032.4467964
ACGCAGA303100.032.0187537
CGCAGAG304250.031.956338
TATCAAC304350.031.1458842
TACAACG3450.031.0074942
ATCAACG306250.030.913843
ACATGGG254800.030.905073
TCAACGC311250.030.760934
CAACGCA313850.030.6190745
TATCACG3300.030.6159862
GCAGAGT320650.030.2477539
GTACATG273450.030.1942651
TACATGG273700.030.0955092
ATAACGC6050.027.5052683
CATGGGT28450.027.1565364
CATGGGC59150.026.6259064