FastQCFastQC Report
Fri 10 Feb 2017
SRR3557668.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557668.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6164776
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1000671.6232057742244No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT550510.8929927056554854No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT359190.5826489072757876No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190390.3088352277519897No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA143830.23330936922931184No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118590.19236708681710415No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT112270.18211529502450696No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95440.15481503301985344No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93510.15168434343762044No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA92810.150548860169453No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC79230.1285204847670053No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76660.12435163905387642No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC70440.11426205915673172No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA65550.10632989746910512No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCAG931450.036.754326
GTATCAA923250.036.606631
CGCATAG8700.036.241891
CATGGGT116600.036.1913874
CATGGGG201300.034.1908764
CGCAGAG1037500.032.997418
ACGCAGA1037000.032.950277
ACATGGG857550.031.6392923
GCAGAGT1085150.031.5594149
TATCAAC1070050.031.4770932
ATGGGTG67800.031.3833125
ATCAACG1085550.031.0769213
TACATGG906650.030.8108482
GTACATG905850.030.7948571
TCAACGC1105950.030.7723944
GACCGTT11800.030.7250987
CAACGCA1113450.030.6184985
GGACCGT17600.029.0423156
CATGGGC185450.028.9084724
TACAACG9350.027.3339442
Produced by FastQC (version 0.11.5)