FastQCFastQC Report
Fri 10 Feb 2017
SRR3557666.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557666.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1567747
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174231.1113400312678001No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94170.6006708990672602No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87160.5559570517436806No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT40520.25846007040676844No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36900.23536961001998408No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA36830.23492310940477004No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35180.22439845204615286No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA34640.22095401872878723No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT24670.15735957396187011No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA21260.13560861542072797No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT20290.12942139260990454No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19490.12431852843602954No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA16840.10741529086006861No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG16310.10403464334487644No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG5500.075.718571
TAACGCA5900.051.426294
ATAGTAC8250.050.4790424
GCATAGT8700.048.5518882
TAGTACT9000.047.594535
GTATAAC6300.047.2167931
TACAACG4200.045.3281172
ACAACGC4750.042.5845763
AGATTAC9500.041.9583322
ATAACGC7700.041.7224733
TACGCAG3450.037.9376645
GATTACT11150.035.7492523
CATAGTA11950.035.34743
CATGGGC57800.033.7608264
GTACAAC6350.033.7284051
CATGGGT22650.031.7822174
ATCTACG3750.031.7296872
ACATGGG248350.030.6868273
AACGCAG260100.030.3984646
CATGGGG49500.030.167244
Produced by FastQC (version 0.11.5)