FastQCFastQC Report
Fri 10 Feb 2017
SRR3557665.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557665.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2216910
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT244831.1043750084577182No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133990.6043998177643657No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111120.5012382099408635No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52920.23871063777961216No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48660.219494702085335No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA45820.2066840782891502No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA42290.19076101420445574No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT31880.14380376289520097No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT28110.12679811088406837No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT27740.12512912116414288No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27100.12224222002697448No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC25390.11452878105110266No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG25070.11308533048251847No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22360.10086110847982101No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA22190.10009427536526065No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG6950.074.482521
TAACGCA6950.050.506584
GCATAGT11750.045.0653462
TAGTACT11900.044.9962465
ATAGTAC11800.044.3691834
ACGCATA2900.043.0865021
AGATTAC12200.041.940072
TACAACG4400.041.9179082
AAACGCA7400.041.8073235
GTATAAC9400.039.244951
ATAACGC11400.037.0547643
ACAACGC5300.037.0449183
TATCACG3650.035.8608322
TCACGCA4300.034.5901374
CATAGTA15950.033.5716253
GTGTTAT11400.032.88181
GATTACT15600.032.7992863
CATGGGG71100.032.3833734
CATGGGC65600.030.2916834
ACATGGG312650.030.1049963
Produced by FastQC (version 0.11.5)