FastQCFastQC Report
Fri 10 Feb 2017
SRR3557662.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557662.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3394062
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT246080.7250309511140338No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147940.4358788967319984No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124220.36599213567695577No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA79320.23370227179114583No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT63740.18779857291941043No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA54380.1602209977307427No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT53680.15815857223586371No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51490.15170612675902798No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47490.13992083821686227No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG44030.12972656362788895No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT42880.1263382931720163No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC40190.11841268662740986No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG39100.11520119549966973No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA37190.1095737202207856No Hit
CTGCACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT34680.10217845166057661RNA PCR Primer, Index 39 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG8600.060.878631
ACAACGC7050.049.7894333
TAACGCA8500.045.495534
AGATTAC17350.043.2061272
TACAACG8650.039.204242
GTATAAC10450.038.714471
TATCACG6500.037.527082
ATAGTAC14400.037.183844
TAGTACT14950.035.8158765
GATTACT21000.035.696493
TCACGCA7400.035.37494
AAACGCA11200.033.996665
GCATAGT15800.033.5125272
ACGCATA3200.033.4659541
GTACAAC11150.033.0824361
ATAACGC15500.031.8581563
CATGGGT38150.031.0336464
CATGGGC119850.029.9828664
CATGGGG91500.029.5845224
ATTACTT25600.029.0498814