FastQCFastQC Report
Fri 10 Feb 2017
SRR3557661.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557661.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15718741
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1678031.0675346072563954No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT905590.5761212046181052No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT730400.46466825810031476No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT283630.1804406599739763No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT253890.16152056961813926No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT198180.12607879982245396No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC187400.11922074420591318No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC184510.11738217456474409No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC181350.11537183544152806No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA173300.11025056014346186No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA172470.10972252803198425No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163390.10394598396907234No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC162630.10346248468627353No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1070050.071.009771
GGTATCA429800.069.796521
TATCAAC1484250.051.0827062
ATCAACG1545150.048.8922353
TCAACGC1597400.047.4120254
CAACGCA1617800.046.828885
AACGCAG1639300.046.534086
ACGCAGA1918900.039.6234477
CGCAGAG1926750.039.446588
GCAGAGT2023100.037.5885249
GACCGTT41150.036.1446467
ACGGTAC47600.034.8716473
GGACCGT55300.034.319376
AGAGTAC1968350.031.5930610-11
GTACTGG139600.029.4929311
TACTGGT130900.028.5881582
GGTTCAC129100.027.8806516
AGTACTT1161800.027.84208712-13
TAGGACC68150.027.3245034
AGTACAT1101400.027.15693912-13
Produced by FastQC (version 0.11.5)