FastQCFastQC Report
Fri 10 Feb 2017
SRR3557657.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557657.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8259600
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT509500.616857959223207No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT302930.36676110223255365No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT245010.29663664099956416No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA142820.17291394256380455No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT134690.16307085088866288No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107920.13066008039130225No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA90240.10925468545692285No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG20800.056.924241
TAACGCA20800.040.616364
GTATAAC23100.037.347671
TACAACG14750.037.108362
ATAGTAC33750.035.432214
AGATTAC39350.035.3791242
TAGTACT34650.034.6835945
TATCACG13000.034.323682
GCATAGT38300.031.0675622
CATGGGG205950.030.6499334
GATTACT45350.030.4359343
ACATGGG875000.030.3727323
CATGGGT114650.029.993684
CATGGGC215750.029.3956034
GTACATG957150.029.2038781
TACATGG960050.029.0887582
ACAACGC18850.028.7214263
AACGCAG891350.028.7076156
AAACGCA27800.028.2491325
GTATCAA903950.026.7364061
Produced by FastQC (version 0.11.5)