FastQCFastQC Report
Fri 10 Feb 2017
SRR3557655.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557655.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6956320
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1055301.5170377440945788No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT543550.781375784897762No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT448160.6442486832118132No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202100.29052717528808336No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102230.14695988683671826No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC97940.14079283299215675No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC95660.13751523794190032No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC92120.13242634036387055No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA88870.1277543298755664No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC87070.12516675483589024No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT86420.12423235273822941No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA945550.045.531671
AACGCAG1085750.039.7757536
TATCAAC1123850.038.25792
ATCAACG1139900.037.5730823
TCAACGC1167650.036.853374
GACCGTT13150.036.6463437
CAACGCA1174300.036.6446725
CGCAGAG1245050.034.576678
ACGCAGA1245250.034.5758977
GGACCGT16850.034.2486346
GCAGAGT1289350.033.416369
ACGGTAC15600.033.179213
GGTATCA571500.030.6285171
GTACTGG50000.030.4627761
GGTTCAC43400.029.6097986
CATGGGT109700.028.8520324
TACTGGT48100.028.5718462
AGTACTT653650.027.4112-13
AGAGTAC1274350.026.9282410-11
GAGTACT612950.025.5805212-13
Produced by FastQC (version 0.11.5)