FastQCFastQC Report
Fri 10 Feb 2017
SRR3557652.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557652.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1153768
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100440.8705389645058625No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51990.4506105213526463No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51360.44515015150359516No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT27120.23505592112105728No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27030.23427586828547853No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA25910.22456854410938765No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21260.1842658142711533No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA20530.17793871904923694No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT19370.16788470472399997No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT18450.15991083129363964No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG14450.1252418163790294No Hit
CTGCACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT14150.12264164026043363RNA PCR Primer, Index 31 (95% over 22bp)
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA13440.11648789011309033No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12920.111980918174191No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG12620.10938074205559523No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG3850.075.73431
TAACGCA3100.049.8970454
ACAACGC2300.049.1460883
GCATAGT6100.047.789162
TACAACG2250.047.5941052
TAGTACT6200.047.018375
ATAGTAC6150.046.4332734
GTATAAC3850.046.367941
GTACAAC3550.040.2290881
TATCACG1508.54925E-1139.6617552
ATAACGC5150.035.8111043
CATAGTA8350.035.6243323
AGATTAC6450.033.2051932
CATGGGC39700.032.3687864
CATGGGG35600.030.7494
ATGGGCA13450.030.078065
GATTACT7250.029.5411683
GGTTTCA5750.028.9766061
GTACTTT129800.028.7441961
CTAACGT1055.909943E-428.3298264
Produced by FastQC (version 0.11.5)