FastQCFastQC Report
Fri 10 Feb 2017
SRR3557649.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557649.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3080591
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT371461.2058075869208214No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205680.6676640943247578No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187470.6085520603027146No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73650.23907750168717626No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32180.10446047527893187No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA320650.047.7995221
AACGCAG376300.040.6179246
GGTATCA186650.040.0697141
TATCAAC387050.039.4744262
ATCAACG389600.039.0938843
TCAACGC396900.038.404834
CAACGCA400750.038.0655675
ACGCAGA430650.035.4226767
CGCAGAG432950.035.2070168
GCAGAGT452200.033.7082679
ACGGTAC6450.030.439823
AGAGTAC443750.028.26982110-11
AGTACTT241350.026.7590312-13
GAGTACT225800.025.78248212-13
CAGAGTA450600.025.13329910-11
GTACTGG20850.024.5415151
GGACCGT8050.024.3896686
TACTTTT270800.023.80499514-15
AGTACAT265800.022.9097712-13
GTACTTT257300.022.42950814-15
Produced by FastQC (version 0.11.5)