FastQCFastQC Report
Fri 10 Feb 2017
SRR3557647.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557647.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1041318
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88780.8525733733595309No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45600.43790657608914857No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42520.4083286757743552No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA28490.27359557791183864No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT27340.2625518813657307No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27190.2611113992075427No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT21070.2023397271534728No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA18540.1780435947520354No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18220.17497056614790102No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT17950.17237769826316265No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC17240.1655594160477395No Hit
CTGCACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT16180.1553800087965444TruSeq Adapter, Index 10 (95% over 21bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG15160.14558473012086606No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG13680.13137197282674457No Hit
GCACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT13470.12935529780528138TruSeq Adapter, Index 10 (95% over 23bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12060.1158147655183143No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA10850.10419487610893118No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA10510.1009297832170384No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG2700.066.10971
TAACGCA3150.047.2189454
ATCACGC2050.043.5335663
TACAACG2250.042.3081742
TATCACG2150.041.5087472
TACGCAG2200.040.5653655
TCACGCA2400.039.6639144
ATAGTAC4750.038.8288844
AGATTAC5850.037.6298682
ACAACGC2750.036.7792633
GTATAAC4700.036.7119831
TAGTACT5050.036.5222175
CATGGGG31200.036.231464
CTACGCA3000.035.6975254
TCTACGC3200.035.3256723
GCATAGT5200.034.324542
ATAACGC5400.034.1550373
GATTACT6500.033.866883
GTACAAC3450.032.7674141
CATGGGC35750.032.6187174
Produced by FastQC (version 0.11.5)