FastQCFastQC Report
Fri 10 Feb 2017
SRR3557643.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557643.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9953103
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT796470.8002228048880837No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT450050.45217054420114006No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT360380.36207803737186284No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157630.15837272054755186No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA531700.062.6861081
GGTATCA246200.056.887041
TATCAAC711650.046.7326742
ATCAACG724550.045.777473
TCAACGC733750.045.3005644
AACGCAG744450.044.9131856
CAACGCA747750.044.492195
ACGCAGA859350.038.8318827
CGCAGAG873500.038.196038
GCAGAGT926550.035.983419
AGAGTAC896350.030.27300310-11
AGTACTT500700.027.15668112-13
CAGAGTA921000.025.75165710-11
GAGTACT466000.025.22111112-13
AGTACAT545850.024.89406612-13
TACTTTT558800.024.19471714-15
GTACATG663750.023.7001881
GGACCGT24950.023.6068336
TACATGG678600.023.0840642
ACATGGG671600.022.9260273
Produced by FastQC (version 0.11.5)