FastQCFastQC Report
Fri 10 Feb 2017
SRR3557642.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557642.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences872736
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105611.2101024823085103No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57440.658160085065816No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54840.6283687163128369No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34480.395079382539508No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT32860.3765170681626517No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA27550.3156739265940674No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA24660.28255967440325597No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22450.2572370109632237No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT21620.24772668939977266No Hit
CTGCACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT16310.18688354783118835RNA PCR Primer, Index 33 (95% over 22bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16130.1848210684559821No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT15400.17645656876764565No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA14710.16855039782935505No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC13850.15869632970336964No Hit
GCACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT13340.15285263814028527RNA PCR Primer, Index 33 (95% over 24bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG12970.14861309720236132No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG12700.14551937813955193No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA12490.1431131522018113No Hit
GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAA11090.12707164595020717No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA9810.11240512594874051No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCC9550.10942598907344261No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG9320.10679059876067906No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG2750.064.903091
GTATAAC2700.063.90151
TATCACG1400.050.9923632
AGATTAC5300.047.1438832
TAACGCA4000.044.6183174
ATAACGC4250.043.39353
TAGTACT4600.041.385115
GTGTTAT5500.038.9418531
ATAGTAC4750.038.8257644
TACGCAG1850.038.5888185
GCATAGT4900.037.6372222
GATTACT6850.037.3447723
GTATCAC2100.036.829931
GAGTTCT5400.036.357751
ATCACGC2150.035.9713553
GTACTTT141850.035.608621
TCACGCA2200.035.1538244
ATCTACG1703.274181E-1034.994762
TACAACG1902.910383E-1134.442212
ACGCATA1408.068491E-833.9968571
Produced by FastQC (version 0.11.5)