##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3557642.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 872736 Sequences flagged as poor quality 0 Sequence length 125 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.170510899057675 33.0 32.0 34.0 30.0 34.0 2 32.19676282403842 33.0 32.0 34.0 30.0 34.0 3 32.22709616653833 33.0 32.0 34.0 30.0 34.0 4 32.147536024639756 33.0 33.0 34.0 30.0 34.0 5 32.15132525758076 33.0 33.0 34.0 30.0 34.0 6 35.348906198438016 38.0 36.0 38.0 29.0 38.0 7 35.68682511091556 38.0 36.0 38.0 30.0 38.0 8 35.91410231731016 38.0 37.0 38.0 31.0 38.0 9 36.07733380999523 38.0 37.0 38.0 32.0 38.0 10-11 36.0551673129102 38.0 37.0 38.0 31.5 38.0 12-13 36.063249940417265 38.0 37.0 38.0 32.0 38.0 14-15 36.15834456238771 38.0 37.0 38.0 33.0 38.0 16-17 36.14055796942031 38.0 37.0 38.0 33.0 38.0 18-19 36.16113578447549 38.0 37.0 38.0 33.0 38.0 20-21 36.041318909727565 38.0 37.0 38.0 31.5 38.0 22-23 36.08593492190078 38.0 37.0 38.0 32.0 38.0 24-25 36.07544148516848 38.0 37.0 38.0 32.0 38.0 26-27 35.97290188556448 38.0 37.0 38.0 31.5 38.0 28-29 35.88812653540131 38.0 37.0 38.0 31.0 38.0 30-31 35.65194743885894 38.0 37.0 38.0 30.0 38.0 32-33 35.415615948007186 38.0 36.5 38.0 28.5 38.0 34-35 35.10804470080299 38.0 36.0 38.0 26.5 38.0 36-37 34.808185407729255 38.0 36.0 38.0 25.0 38.0 38-39 34.5668443836395 38.0 35.5 38.0 24.0 38.0 40-41 34.32871337953287 38.0 35.0 38.0 16.0 38.0 42-43 34.03533485498478 38.0 35.0 38.0 15.5 38.0 44-45 33.61532754464122 38.0 34.0 38.0 15.0 38.0 46-47 33.21401832856671 38.0 33.0 38.0 15.0 38.0 48-49 32.831321843031574 38.0 31.5 38.0 15.0 38.0 50-51 32.51219039892935 38.0 29.5 38.0 15.0 38.0 52-53 32.2711364032193 38.0 28.5 38.0 15.0 38.0 54-55 32.05074157591758 38.0 26.5 38.0 15.0 38.0 56-57 31.88016020881458 38.0 26.0 38.0 15.0 38.0 58-59 31.74079446705533 38.0 26.0 38.0 15.0 38.0 60-61 31.62143534814652 38.0 25.0 38.0 15.0 38.0 62-63 31.501452902137643 38.0 25.0 38.0 15.0 38.0 64-65 31.373806626517066 38.0 25.0 38.0 15.0 38.0 66-67 31.262075816741834 38.0 25.0 38.0 15.0 38.0 68-69 31.103510110732223 38.0 24.0 38.0 14.0 38.0 70-71 30.93835363729696 38.0 24.0 38.0 14.0 38.0 72-73 30.73923271183955 38.0 24.0 38.0 14.0 38.0 74-75 30.59414129817035 38.0 23.0 38.0 14.0 38.0 76-77 30.461240856341437 38.0 21.0 38.0 14.0 38.0 78-79 30.329152802221977 38.0 16.0 38.0 14.0 38.0 80-81 30.21393296483702 38.0 16.0 38.0 14.0 38.0 82-83 30.072748230851026 37.0 15.5 38.0 14.0 38.0 84-85 29.97283313625197 37.0 15.0 38.0 13.0 38.0 86-87 29.8726401798482 37.0 15.0 38.0 13.0 38.0 88-89 29.74568483481832 37.0 15.0 38.0 13.0 38.0 90-91 29.596008987826785 37.0 15.0 38.0 13.0 38.0 92-93 29.536497864188025 37.0 15.0 38.0 13.0 38.0 94-95 29.477914856268104 37.0 15.0 38.0 13.0 38.0 96-97 29.377428569464307 37.0 15.0 38.0 13.0 38.0 98-99 29.3003972564441 36.5 14.0 38.0 13.0 38.0 100-101 29.18791937080629 36.0 14.0 38.0 13.0 38.0 102-103 29.08199444047226 36.0 14.0 38.0 13.0 38.0 104-105 28.981630183698165 36.0 14.0 38.0 13.0 38.0 106-107 28.909739027609724 35.5 14.0 38.0 13.0 38.0 108-109 28.757310343563233 35.0 14.0 38.0 13.0 38.0 110-111 28.686860631393685 35.0 14.0 38.0 13.0 38.0 112-113 28.59773001228321 35.0 14.0 38.0 13.0 38.0 114-115 28.5098328704213 35.0 14.0 38.0 13.0 38.0 116-117 28.299433047336194 34.5 14.0 38.0 13.0 38.0 118-119 28.15604604370623 34.0 14.0 38.0 13.0 38.0 120-121 28.08728871044623 34.0 14.0 38.0 13.0 38.0 122-123 27.95492680489862 34.0 14.0 38.0 13.0 38.0 124-125 26.78959215616177 29.5 14.0 37.0 13.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.0241306874038969 1101 2 0.0977657384577455 1101 3 0.24882628214010793 1101 4 0.2937294476273635 1101 5 0.24432051767827545 1101 6 0.3584837213141796 1101 7 0.5689325288759548 1101 8 0.4146955026347854 1101 9 0.3906749762909101 1101 10-11 0.15218285505897455 1101 12-13 0.16620793137503398 1101 14-15 0.1070148201976977 1101 16-17 0.15461888061915374 1101 18-19 0.33652474347145755 1101 20-21 0.13273151535241823 1101 22-23 0.3753657562109609 1101 24-25 0.34930200597776206 1101 26-27 0.22213003424150912 1101 28-29 0.10210643664056818 1101 30-31 0.1476008905897146 1101 32-33 0.2744641511458781 1101 34-35 -0.09251891465328299 1101 36-37 -0.11741490048412118 1101 38-39 -0.020325737360863627 1101 40-41 -0.05143170749911974 1101 42-43 0.041165099116462045 1101 44-45 0.04270038001021703 1101 46-47 0.08318851261235238 1101 48-49 -0.12314767061701559 1101 50-51 -0.049120134717036024 1101 52-53 -0.06033626539656467 1101 54-55 0.16843652397859898 1101 56-57 0.21109233839485952 1101 58-59 0.1865114301378945 1101 60-61 -0.051308235931532664 1101 62-63 0.1292486739109684 1101 64-65 0.19729505802035874 1101 66-67 0.1132394845403617 1101 68-69 -0.2524343754637748 1101 70-71 -0.2247246143908761 1101 72-73 -0.13837581765162454 1101 74-75 -0.00823829626397199 1101 76-77 -0.06955663689405611 1101 78-79 -0.31200445547797884 1101 80-81 0.014305337459521894 1101 82-83 -0.05412591944242706 1101 84-85 -0.002405416187027498 1101 86-87 0.013455838640126672 1101 88-89 0.3453172222572434 1101 90-91 -0.11325527028211724 1101 92-93 0.08879403855462442 1101 94-95 0.10147016097868189 1101 96-97 -0.17257871217843856 1101 98-99 0.23295444558780076 1101 100-101 0.04472144250128096 1101 102-103 -9.050183701440062E-4 1101 104-105 0.07205820164306331 1101 106-107 0.0775593838957711 1101 108-109 0.02051021622943594 1101 110-111 -0.3750003626769285 1101 112-113 -0.2628989386131657 1101 114-115 -0.08548347085686459 1101 116-117 -0.13348858753036197 1101 118-119 0.12056885179925914 1101 120-121 -0.009115641204456182 1101 122-123 -0.14487199099788484 1101 124-125 -0.13630253686538296 1102 1 0.013675283207810196 1102 2 0.25871333714523104 1102 3 0.07851953062375117 1102 4 0.19289420849044348 1102 5 0.18406646577413 1102 6 0.34700912650459514 1102 7 0.23008147985537164 1102 8 0.44331984388963264 1102 9 0.4374135663276988 1102 10-11 0.33606970004257875 1102 12-13 0.37937786276611263 1102 14-15 0.23033564151617725 1102 16-17 0.15578100650161986 1102 18-19 0.21393102765163263 1102 20-21 0.4372532415133037 1102 22-23 0.3725182370700608 1102 24-25 0.19133367546170632 1102 26-27 0.09526068950649602 1102 28-29 0.046114341582402574 1102 30-31 0.060569469688850575 1102 32-33 -0.06971154737257024 1102 34-35 -0.3262528810448515 1102 36-37 -0.4253049273310907 1102 38-39 0.0601977785981731 1102 40-41 0.0058753920335377074 1102 42-43 -0.2902185142871403 1102 44-45 0.04998386415061162 1102 46-47 -0.1797726089944902 1102 48-49 0.08297244472553444 1102 50-51 0.23839975192908724 1102 52-53 0.3140136898584771 1102 54-55 0.1985610640999056 1102 56-57 0.305751530997032 1102 58-59 0.13900347547944492 1102 60-61 0.12143711556494097 1102 62-63 0.27042273178110676 1102 64-65 0.3281752906941193 1102 66-67 0.286458386797495 1102 68-69 0.6354136408432787 1102 70-71 0.32215815383026225 1102 72-73 0.49299163785200406 1102 74-75 0.4167057725438248 1102 76-77 0.19900630434090516 1102 78-79 0.2630539615449905 1102 80-81 -0.04753094572631866 1102 82-83 0.4000415758344964 1102 84-85 0.1330972187935835 1102 86-87 0.21997741565116158 1102 88-89 0.02428087941146373 1102 90-91 0.21319769779981357 1102 92-93 0.26667263058007507 1102 94-95 0.246922333594771 1102 96-97 0.2170441872945652 1102 98-99 0.07178312213149951 1102 100-101 0.09820160557177715 1102 102-103 0.09905023667609214 1102 104-105 0.3405092804936132 1102 106-107 -0.048413818848583645 1102 108-109 1.5001935163994062E-4 1102 110-111 0.17429739013206103 1102 112-113 0.1469635949455501 1102 114-115 -0.03524234527269243 1102 116-117 -0.05785345776999762 1102 118-119 0.12614705603511212 1102 120-121 -0.08637526136951479 1102 122-123 0.05912895361780457 1102 124-125 0.023110061324931763 1103 1 0.20791260972995218 1103 2 0.04550825894650501 1103 3 0.2399234588042347 1103 4 0.23316497865036467 1103 5 0.05643960517294744 1103 6 0.14537091670804614 1103 7 0.4633212850820456 1103 8 0.241701765703489 1103 9 0.38976078627812427 1103 10-11 0.37556729054045235 1103 12-13 0.32693690500474304 1103 14-15 0.12039754621816456 1103 16-17 0.2399154101655725 1103 18-19 0.260841454567597 1103 20-21 0.3281216688570652 1103 22-23 0.2018833364069721 1103 24-25 0.11485872044327294 1103 26-27 0.1219419935315571 1103 28-29 0.14772612864028645 1103 30-31 -0.043131601061183744 1103 32-33 -0.08475678058469072 1103 34-35 0.13030499104392845 1103 36-37 0.15744165344069927 1103 38-39 0.3710776557953466 1103 40-41 0.2656404813046507 1103 42-43 0.18096308542094874 1103 44-45 0.09987565674470034 1103 46-47 0.30896123853372615 1103 48-49 0.4019615862381727 1103 50-51 0.4213501652562073 1103 52-53 0.4498529892784404 1103 54-55 0.3831157151310194 1103 56-57 0.3880011126370029 1103 58-59 0.49041457554613643 1103 60-61 0.4639034894844407 1103 62-63 0.33382346406843766 1103 64-65 0.2827067903732683 1103 66-67 0.3445475195025587 1103 68-69 0.23936950228747023 1103 70-71 0.3308650753295659 1103 72-73 0.20441632675571242 1103 74-75 0.21118279105232673 1103 76-77 0.061280924671311965 1103 78-79 0.42902273238435384 1103 80-81 0.3187064562893589 1103 82-83 0.10339285985377344 1103 84-85 0.08122686636457743 1103 86-87 0.14690218633953833 1103 88-89 0.03681091954886284 1103 90-91 -0.010633725127568994 1103 92-93 -0.018234323633087968 1103 94-95 -0.025911316392019046 1103 96-97 0.08517270246247577 1103 98-99 0.14273949944820785 1103 100-101 0.07264263882668232 1103 102-103 0.021776308369791053 1103 104-105 0.49896078218592876 1103 106-107 0.26110780375921294 1103 108-109 0.17621919659788787 1103 110-111 0.19771800238466142 1103 112-113 -0.19150602519612647 1103 114-115 0.019768538379182132 1103 116-117 0.023083794516825407 1103 118-119 0.16437069306217822 1103 120-121 0.2472935988038678 1103 122-123 0.3255462819014596 1103 124-125 -0.12133298509101209 1104 1 0.1184745167240564 1104 2 -0.0757635003627044 1104 3 0.13194817670983383 1104 4 0.10707042509675802 1104 5 0.09524393911517848 1104 6 0.14109625181721697 1104 7 0.23586992536161233 1104 8 0.16809493814473342 1104 9 0.2062577436641675 1104 10-11 0.1646143763400616 1104 12-13 0.08340625750524566 1104 14-15 0.26899516594784956 1104 16-17 0.10787266103644555 1104 18-19 -0.1573930232647882 1104 20-21 0.14012942203515877 1104 22-23 -0.03165325122737528 1104 24-25 0.023634246591655028 1104 26-27 -0.20734423540049107 1104 28-29 -0.11275932547000167 1104 30-31 -0.5098866876891819 1104 32-33 -0.18992568999329507 1104 34-35 0.0608568779358265 1104 36-37 0.1269671495163749 1104 38-39 0.22557411465651 1104 40-41 0.509239334945434 1104 42-43 0.29927841853354664 1104 44-45 0.6901440045067346 1104 46-47 0.24795394543705385 1104 48-49 0.44349106555249307 1104 50-51 0.5321876250610913 1104 52-53 0.5041053941649736 1104 54-55 0.6415270824905406 1104 56-57 0.620382536260383 1104 58-59 0.6881106961048253 1104 60-61 0.7472001775182271 1104 62-63 0.7589864457433073 1104 64-65 0.3341391813457548 1104 66-67 0.577656888670159 1104 68-69 0.37362833095240333 1104 70-71 0.5639738244413337 1104 72-73 0.532832617358519 1104 74-75 0.5632852143545186 1104 76-77 0.5283330180418027 1104 78-79 0.3762807834992721 1104 80-81 0.4746728195007037 1104 82-83 0.5505417811426447 1104 84-85 0.39069673103119484 1104 86-87 0.5426683211223136 1104 88-89 0.5192831813513159 1104 90-91 0.31970969899433754 1104 92-93 0.260587421476842 1104 94-95 0.5378688663756357 1104 96-97 0.5469090572038553 1104 98-99 0.3470627767160934 1104 100-101 0.3168379861757593 1104 102-103 0.4460147868023867 1104 104-105 0.4602816995213246 1104 106-107 0.3195214033197189 1104 108-109 0.48039626470110264 1104 110-111 0.39610982558442487 1104 112-113 0.43072372037016393 1104 114-115 0.2942598135735963 1104 116-117 0.3921652066071708 1104 118-119 0.2139057131953912 1104 120-121 0.6155670781479508 1104 122-123 0.5376593205594595 1104 124-125 0.42922326699392244 1105 1 0.0921029720694051 1105 2 0.07226753587591617 1105 3 -0.05161643286240292 1105 4 0.026891101441776755 1105 5 0.27847739102431746 1105 6 0.15883277138578933 1105 7 0.11117304144839579 1105 8 0.2354125254653141 1105 9 0.04055072205346022 1105 10-11 0.05620831295006923 1105 12-13 0.05616230825426527 1105 14-15 0.023114161934785216 1105 16-17 0.08885602125408099 1105 18-19 0.11758000340942942 1105 20-21 0.15017097698062543 1105 22-23 0.11074276348599454 1105 24-25 -0.08235757060931803 1105 26-27 -0.20804928074772278 1105 28-29 -0.043456572588389974 1105 30-31 -0.022144862476331184 1105 32-33 0.37518952082594836 1105 34-35 0.3046387696650754 1105 36-37 0.3760119555042678 1105 38-39 0.29866737648826813 1105 40-41 0.41502854062995453 1105 42-43 0.16741001275502043 1105 44-45 0.34537326887381425 1105 46-47 0.45380760223664396 1105 48-49 0.3837455940445125 1105 50-51 0.30114106002066166 1105 52-53 0.5318033498895502 1105 54-55 0.48641888651641807 1105 56-57 0.6466953271361042 1105 58-59 0.39082013552255646 1105 60-61 0.6463982774566226 1105 62-63 0.6201993373906554 1105 64-65 0.4030431206976104 1105 66-67 0.6741199111781917 1105 68-69 0.4493833170117334 1105 70-71 0.4107030193721286 1105 72-73 0.4249304121333459 1105 74-75 0.3835235766552465 1105 76-77 0.3801364029716332 1105 78-79 0.3813229259825306 1105 80-81 0.3957166426981189 1105 82-83 0.2878536355187329 1105 84-85 0.4566879072818466 1105 86-87 0.4175423565811087 1105 88-89 0.3285470499137695 1105 90-91 0.3588201311728909 1105 92-93 0.1801624002008495 1105 94-95 0.3635570867770852 1105 96-97 0.3770180543658661 1105 98-99 0.17437473791723335 1105 100-101 0.25381476597015507 1105 102-103 0.3522686814657625 1105 104-105 0.634321075235718 1105 106-107 0.7144732074168161 1105 108-109 0.44330126518181245 1105 110-111 0.4984589084913438 1105 112-113 0.5496449297721924 1105 114-115 0.4897176123365625 1105 116-117 0.5339327339276778 1105 118-119 0.5688942651105862 1105 120-121 0.49081322087333845 1105 122-123 0.6751200178421861 1105 124-125 0.3723531698187337 1106 1 0.27418771999802516 1106 2 0.17705292608548007 1106 3 0.14099071661441087 1106 4 0.13361519233045982 1106 5 0.25252251762123734 1106 6 0.2651688918891608 1106 7 0.09782547869677671 1106 8 0.2206765583080994 1106 9 0.0579967256706766 1106 10-11 0.009762962090142935 1106 12-13 0.10207002656243702 1106 14-15 0.010083767851277514 1106 16-17 0.0699997395300258 1106 18-19 0.16489109233984323 1106 20-21 0.2725077099476323 1106 22-23 0.2921546072893477 1106 24-25 0.17702020317746303 1106 26-27 0.04867371645589458 1106 28-29 0.13394547640178445 1106 30-31 0.2498615200394454 1106 32-33 0.25275493182703457 1106 34-35 0.27809848694172956 1106 36-37 0.1653146794708249 1106 38-39 0.2903248619566483 1106 40-41 0.0901142797826111 1106 42-43 0.11843594690596859 1106 44-45 0.2893996186250831 1106 46-47 -0.06519603915898387 1106 48-49 0.12312619989998552 1106 50-51 -0.15101539911063355 1106 52-53 -0.15072520392493516 1106 54-55 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86-87 0.42789359569492547 2313 88-89 0.4093727672398515 2313 90-91 0.5042042353268315 2313 92-93 0.38887941511016777 2313 94-95 0.4952074073600876 2313 96-97 0.42148285938455743 2313 98-99 0.3006583184586731 2313 100-101 0.5283809512821129 2313 102-103 0.47172954882622875 2313 104-105 0.43754188758421364 2313 106-107 0.3391413876808862 2313 108-109 0.3604932730801771 2313 110-111 0.5209823135476839 2313 112-113 0.6174648500970399 2313 114-115 0.8074059572523744 2313 116-117 0.7961173851279923 2313 118-119 0.8194618641378888 2313 120-121 0.7225799424751429 2313 122-123 0.46842177307735255 2313 124-125 0.4077555984894943 2314 1 -0.18930178707249823 2314 2 -0.03303824229781327 2314 3 -0.15320144097897526 2314 4 -0.008819307356311867 2314 5 -0.08365011085665941 2314 6 -0.16717381420252764 2314 7 -0.1865958690347682 2314 8 -0.2181495940510132 2314 9 -0.35995860669815727 2314 10-11 -0.48442381868184015 2314 12-13 -0.3636477353294012 2314 14-15 -0.35797778800076685 2314 16-17 -0.5439057209447142 2314 18-19 -0.48634889020424765 2314 20-21 -0.15293328318879418 2314 22-23 0.014810031814405988 2314 24-25 -0.2595564371594463 2314 26-27 -0.26586408322374666 2314 28-29 -0.10344681523699961 2314 30-31 -0.12074339737633721 2314 32-33 0.08996916314789871 2314 34-35 -0.01286195090659703 2314 36-37 -0.07729312892426776 2314 38-39 0.03397052192396188 2314 40-41 -0.2518184249211046 2314 42-43 -0.16612857432559736 2314 44-45 -0.11460848912142296 2314 46-47 -0.020137025418080157 2314 48-49 0.016392543874005128 2314 50-51 0.049181167476298526 2314 52-53 0.1015808897642998 2314 54-55 -0.024188801827293105 2314 56-57 0.22856287421032917 2314 58-59 0.15680965198465913 2314 60-61 0.22562934326634476 2314 62-63 -0.05015253865354552 2314 64-65 0.13752899437359645 2314 66-67 -0.023429646060147746 2314 68-69 -1.776813798954766 2314 70-71 -0.9229073129393051 2314 72-73 -0.2104890229634222 2314 74-75 -0.06195441502729793 2314 76-77 -0.1222386761040859 2314 78-79 0.08318842675799942 2314 80-81 -0.03164037529558428 2314 82-83 0.09799516702362254 2314 84-85 -0.15001222385595625 2314 86-87 0.16252362171779566 2314 88-89 0.014242423406965798 2314 90-91 -0.05617549548738765 2314 92-93 -0.32293574602715935 2314 94-95 -0.14933147603492714 2314 96-97 -0.005657281620166543 2314 98-99 -0.05424923846548424 2314 100-101 0.003924879302662276 2314 102-103 -0.013028088002460692 2314 104-105 -0.13171148836487134 2314 106-107 0.13300369040570814 2314 108-109 0.06704057670210162 2314 110-111 0.06744725082714353 2314 112-113 0.0029412799987191818 2314 114-115 0.10470088633971031 2314 116-117 -0.01597761572738321 2314 118-119 -0.05376949181906454 2314 120-121 -0.2611734981486258 2314 122-123 -0.42931920271762536 2314 124-125 0.12754035000656927 2315 1 -0.1612800508931116 2315 2 -0.025119592540718827 2315 3 -0.12426100430828768 2315 4 -0.47237226495485274 2315 5 0.17024716745886082 2315 6 -0.2546478129218315 2315 7 -0.4352538121603473 2315 8 -0.5045236203313266 2315 9 -0.2796216767209643 2315 10-11 -0.5116434059247936 2315 12-13 -0.5444459352249211 2315 14-15 -0.29845765132007074 2315 16-17 -0.2583976041573166 2315 18-19 -0.055614108066528445 2315 20-21 0.011903524066468663 2315 22-23 -0.07410303580881106 2315 24-25 -0.09096804103225509 2315 26-27 -0.03857621048158677 2315 28-29 -0.12322698529258247 2315 30-31 0.012005395629479665 2315 32-33 -0.03639740383290757 2315 34-35 -0.12855102426688347 2315 36-37 -0.20017754680409894 2315 38-39 -0.09674958842978754 2315 40-41 0.1726287436302627 2315 42-43 -0.14411404240349412 2315 44-45 0.0730744796797893 2315 46-47 -0.17761805836651945 2315 48-49 -0.055876961714297124 2315 50-51 -0.09782462307918394 2315 52-53 -0.21523023227066318 2315 54-55 -0.06708457319976091 2315 56-57 -0.2595817758676233 2315 58-59 0.1234107755416467 2315 60-61 0.357903017286727 2315 62-63 0.14329630412663263 2315 64-65 0.3944250756360823 2315 66-67 0.11947078737451378 2315 68-69 0.3434482089405755 2315 70-71 0.29789960775490343 2315 72-73 0.2633520089429098 2315 74-75 0.07152042560559124 2315 76-77 0.11087883117352959 2315 78-79 0.3136567857532455 2315 80-81 0.553941374650595 2315 82-83 0.18889098227432655 2315 84-85 0.23542513928166287 2315 86-87 0.4420046519238454 2315 88-89 0.21518690235981097 2315 90-91 0.1249133976979131 2315 92-93 0.26148940113631625 2315 94-95 0.4291989048418401 2315 96-97 0.17236836246429377 2315 98-99 0.5671008124744006 2315 100-101 0.4342009967921179 2315 102-103 0.35983953274152114 2315 104-105 0.891398628172972 2315 106-107 0.4403905908336583 2315 108-109 0.5885203426665768 2315 110-111 0.5427705442724218 2315 112-113 0.352259316283277 2315 114-115 0.34319759067309974 2315 116-117 0.5680375442019177 2315 118-119 0.3326715858851692 2315 120-121 0.24926746437524727 2315 122-123 0.49696262442682126 2315 124-125 0.6032016429609577 2316 1 -0.2612883651728879 2316 2 -0.09709631255734763 2316 3 -0.12906804373183434 2316 4 -0.028467324886953804 2316 5 -0.02894080454973391 2316 6 -0.2253372052865501 2316 7 -0.20259532120212498 2316 8 -0.7344065275578231 2316 9 -0.4429169029386557 2316 10-11 -0.4081867441081215 2316 12-13 -0.40917468184724015 2316 14-15 -0.31182562748580267 2316 16-17 -0.5811918950185415 2316 18-19 -0.5033789525202081 2316 20-21 -0.30228410151379137 2316 22-23 -0.5517646446219473 2316 24-25 -0.2374171507114582 2316 26-27 -0.357310361385764 2316 28-29 -0.3146986128128404 2316 30-31 -0.2911159235695848 2316 32-33 -0.3209210648874361 2316 34-35 -0.42968315202965357 2316 36-37 -0.21970016857365238 2316 38-39 -0.12318622668933443 2316 40-41 -0.33326538787392934 2316 42-43 -0.20927998157068117 2316 44-45 -0.11183371681435972 2316 46-47 0.10706149543380405 2316 48-49 0.1644265094975097 2316 50-51 0.1358551385781297 2316 52-53 0.08531573876793175 2316 54-55 -0.006455459363415628 2316 56-57 0.04086719524025284 2316 58-59 0.16472720317277023 2316 60-61 0.3514767718769036 2316 62-63 0.39251129754449465 2316 64-65 0.567639930482617 2316 66-67 0.3872647318074094 2316 68-69 0.4411770248485638 2316 70-71 0.23705570835769052 2316 72-73 0.4804073681890806 2316 74-75 0.5725022034757465 2316 76-77 0.6178496619086502 2316 78-79 0.45524827747360774 2316 80-81 0.3318808189795668 2316 82-83 0.5209404522389889 2316 84-85 0.3240027045834033 2316 86-87 0.4881135689889007 2316 88-89 0.3922914544279905 2316 90-91 0.4305539436689507 2316 92-93 0.5223104362641493 2316 94-95 0.32814506988029635 2316 96-97 0.4146714179621114 2316 98-99 0.3590137896794161 2316 100-101 0.6105413279775966 2316 102-103 0.3863932637745684 2316 104-105 0.41976902933697247 2316 106-107 0.4599734911315885 2316 108-109 0.45811432867087376 2316 110-111 0.36308787261718933 2316 112-113 0.24407529355758228 2316 114-115 0.502213041376347 2316 116-117 0.4599588356867095 2316 118-119 0.2767947757971747 2316 120-121 0.3088593718096 2316 122-123 0.21758896683657625 2316 124-125 -0.009044598291726658 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 8.0 15 125.0 16 231.0 17 300.0 18 855.0 19 3624.0 20 12340.0 21 30199.0 22 45020.0 23 48496.0 24 47861.0 25 34650.0 26 23315.0 27 22874.0 28 18695.0 29 18091.0 30 20280.0 31 23067.0 32 28026.0 33 35620.0 34 45566.0 35 62265.0 36 99010.0 37 252218.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.91883495991135 16.381170425729152 11.62347268391676 20.076521930442734 2 14.680269863968027 23.229819785135483 40.21559784402156 21.874312506874933 3 20.500289893598737 34.25419429058568 27.009948048317128 18.23556776749846 4 11.863152201811316 17.212650790158765 36.048472848604845 34.87572415942507 5 12.358949327173395 38.75135206981263 33.39463480365196 15.495063799362008 6 25.234435155648445 33.647288527114725 23.860938473948593 17.257337843288234 7 24.439807685256483 32.90193139735269 25.302496975030248 17.35576394236058 8 26.679545704542956 28.481923514098188 25.994229641036924 18.84430114032193 9 29.76547317860155 14.277628057052763 24.196549701169655 31.760349063176037 10-11 23.34961546217871 25.664863143035237 29.921877864554684 21.063643530231367 12-13 24.945630752025814 24.45980227697723 31.97874271257287 18.615824258424084 14-15 23.469583012503207 22.960952682139848 32.88944193891395 20.680022366443 16-17 19.421337036629634 26.562671873281268 32.59227303560298 21.42371805448612 18-19 19.61732986836798 24.40291222087779 37.02889533604664 18.950862574707585 20-21 21.677345726542736 23.7454969200308 36.057238385949475 18.519918967476993 22-23 20.294461740484536 25.826991982454672 34.49664321866002 19.381903058400777 24-25 18.923034615516826 27.129008971846964 35.01105725711274 18.936899155523474 26-27 19.443462436923127 26.8143249463843 34.73518169749343 19.007030919199146 28-29 19.34365872132797 27.211686878560194 35.229370518786574 18.215283881325263 30-31 20.353033119200244 27.12864944893006 34.826153419521326 17.692164012348364 32-33 18.323455737599723 27.777357617093053 34.88206471544045 19.01712192986678 34-35 18.113159117511742 28.293789958994676 34.36449191953857 19.228559003955006 36-37 18.296032413529165 29.23834852335566 32.872953815762635 19.592665247352535 38-39 18.398175393246067 28.68272879771202 32.9694775968907 19.94961821215121 40-41 17.784665448274676 28.918801239780688 33.03305126985855 20.263482042086085 42-43 18.55669984966817 28.57290177098229 32.503643713562866 20.366754665786676 44-45 18.139849851501484 28.521397077695887 32.25946907197595 21.07928399882668 46-47 17.83965849240047 28.23722655128773 32.186552708576706 21.736562247735094 48-49 17.86030026430547 28.363133827490405 31.69803515554947 22.078530752654657 50-51 17.38299614442064 28.205662986037062 31.685998041555912 22.725342827986385 52-53 17.370001317892974 27.619598787538465 31.477644524154684 23.532755370413877 54-55 17.478371784131554 27.00103256179613 31.787603986765788 23.73299166730653 56-57 18.13984856767297 25.960948933132094 31.54586116678757 24.353341332407368 58-59 17.788491565756956 25.97714769651967 32.27055587102275 23.963804866700627 60-61 18.437385216879573 26.112189344229886 31.73798763307679 23.71243780581375 62-63 17.70806953050067 26.50601098030947 31.962991017048015 23.822928472141847 64-65 17.436235896959936 26.5221658282043 31.657325273553 24.384273001282757 66-67 17.755713067869323 26.646144996883365 31.306660891724412 24.291481043522896 68-69 17.94379972866938 26.78186759799069 31.17437575624244 24.099956917097494 70-71 17.804754244124226 26.599911084222487 31.01241383419499 24.582920837458293 72-73 18.139047776188903 26.535000274997252 30.70625022916437 24.61970171964947 74-75 18.579730869357974 26.010672184944816 30.43921644116892 24.97038050452829 76-77 18.573027811388553 25.92238660946724 29.973669013309866 25.530916565834342 78-79 18.75549994500055 25.80138781945514 29.43381503684963 26.009297198694682 80-81 18.237359293073734 26.037369834634987 28.785451728816046 26.93981914347523 82-83 18.32020221464452 26.30268488981777 28.0885055732776 27.288607322260113 84-85 18.627418403066223 26.178565085621635 27.56100076197242 27.63301574933972 86-87 18.890231417108243 26.02736351329132 27.1509049461375 27.931500123462943 88-89 19.11997336968074 26.343390174856008 26.90011739570603 27.63651905975722 90-91 19.679244580304882 26.582361889809796 26.335062784521458 27.403330745363856 92-93 19.27148481481715 27.294594781978905 25.71032551178332 27.72359489142063 94-95 19.126060378240727 27.46474192210102 26.043531377569145 27.365666322089105 96-97 19.37992625127857 27.515321339854392 25.902361735473022 27.202390673394017 98-99 19.404067851776805 27.86439500818865 25.916767998129618 26.814769141904925 100-101 19.31407476767261 28.136527189052064 26.032758777783442 26.516639265491882 102-103 19.34265703810586 28.687411394051637 26.07728215113754 25.892649416704966 104-105 19.316113950450347 29.03931657231912 26.28898998464465 25.35557949258589 106-107 18.788797546061478 29.696082189065333 26.456381460480717 25.058738804392476 108-109 18.8231971207557 29.95781050016672 26.172958616019404 25.04603376305818 110-111 18.410217070349493 30.191850795573227 26.681738052365233 24.716194081712043 112-113 18.042887040675026 30.388795217789603 26.82534035265021 24.742977388885166 114-115 17.904841784915483 30.366227587724122 27.324815293513733 24.40411533384666 116-117 17.87494607472711 30.42433818722855 27.57565150036867 24.125064237675677 118-119 17.62428729879368 30.647584149158508 27.897726231071022 23.83040232097679 120-121 17.501741649250175 30.981132897004365 28.078078594214055 23.439046859531405 122-123 17.19569262640707 31.627605598944008 27.8204978366883 23.356203937960622 124-125 16.614217513870024 32.77023831159785 27.95515955760396 22.660384616928166 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 209.0 1 170.0 2 431.0 3 716.5 4 586.0 5 512.5 6 705.5 7 1118.5 8 1644.0 9 1902.0 10 1775.5 11 1698.0 12 1818.0 13 1981.0 14 2275.5 15 2638.0 16 2912.0 17 3388.5 18 3942.0 19 4397.0 20 5004.5 21 5691.5 22 6473.5 23 7388.0 24 8341.0 25 9492.5 26 10805.5 27 12417.0 28 14338.5 29 16523.5 30 18770.5 31 20837.5 32 22499.0 33 23105.5 34 22747.0 35 21928.0 36 20970.0 37 20039.0 38 19072.0 39 17986.0 40 17282.5 41 16948.0 42 16573.5 43 17250.5 44 18477.5 45 19756.5 46 22487.5 47 24958.5 48 29451.5 49 34150.5 50 38650.0 51 40307.5 52 34701.0 53 29521.0 54 27029.0 55 24459.0 56 21141.5 57 17829.5 58 15616.5 59 13572.0 60 11206.5 61 9178.5 62 7267.0 63 5454.0 64 3939.5 65 3136.5 66 2387.0 67 1802.0 68 1479.5 69 1215.0 70 1056.5 71 910.0 72 874.0 73 908.0 74 708.0 75 517.5 76 431.5 77 294.5 78 204.0 79 127.0 80 83.5 81 74.0 82 61.5 83 46.0 84 32.5 85 19.5 86 11.0 87 2.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.00515619843801562 2 0.0 3 2.291643750229164E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0028645546877864555 24-25 6.874931250687493E-4 26-27 0.010369687969786968 28-29 0.007447842188244784 30-31 0.008020753125802076 32-33 0.006187438125618744 34-35 0.005098907344259891 36-37 0.001604150625160415 38-39 0.0 40-41 1.145821875114582E-4 42-43 0.0 44-45 0.0 46-47 0.002005188281450519 48-49 0.008192626407069263 50-51 0.011973838594947384 52-53 0.015010266564001026 54-55 0.017072745939207275 56-57 0.01546859531404686 58-59 0.015182139845268216 60-61 0.019077934220657792 62-63 0.009510321563451031 64-65 8.593664063359365E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 1.145821875114582E-4 86-87 5.15619843801562E-4 88-89 0.005328071719282807 90-91 0.012432167344993217 92-93 0.014380064532688006 94-95 0.019478971876947897 96-97 0.020452920470795295 98-99 0.021426869064642688 100-101 0.02366122172111612 102-103 0.022114362189711436 104-105 0.008708246250870823 106-107 0.0018906060939390607 108-109 8.020753125802075E-4 110-111 5.72910937557291E-5 112-113 1.7187328126718732E-4 114-115 0.0 116-117 5.72910937557291E-5 118-119 0.0 120-121 0.0 122-123 0.0 124-125 0.07121282953837128 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 872736.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.53945344645263 #Duplication Level Percentage of deduplicated Percentage of total 1 68.15540106049887 29.674489115978993 2 16.04356088409927 13.970557444571371 3 7.28049091493136 9.50965785773926 4 3.311053995829729 5.766459252404778 5 1.6392153767124293 3.568527079154007 6 0.8479833090856336 2.2152437885581677 7 0.4876302027269787 1.4861806757500884 8 0.33166984598323657 1.1552579055023393 9 0.251956793869509 0.9873054987479053 >10 1.4127464611744698 11.640806656853517 >50 0.1331709720731048 4.007135944480929 >100 0.09306150865656229 7.898933491447929 >500 0.007077917558455404 2.178638304040291 >1k 0.004194321516121721 3.4538858821544025 >5k 5.242901895152151E-4 1.28152508789629 >10k+ 2.6214509475760755E-4 1.2053960147196936 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10561 1.2101024823085103 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5744 0.658160085065816 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5484 0.6283687163128369 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3448 0.395079382539508 No Hit CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT 3286 0.3765170681626517 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 2755 0.3156739265940674 No Hit GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2466 0.28255967440325597 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2245 0.2572370109632237 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 2162 0.24772668939977266 No Hit CTGCACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT 1631 0.18688354783118835 RNA PCR Primer, Index 33 (95% over 22bp) GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1613 0.1848210684559821 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1540 0.17645656876764565 No Hit GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA 1471 0.16855039782935505 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 1385 0.15869632970336964 No Hit GCACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT 1334 0.15285263814028527 RNA PCR Primer, Index 33 (95% over 24bp) GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 1297 0.14861309720236132 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 1270 0.14551937813955193 No Hit GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1249 0.1431131522018113 No Hit GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAA 1109 0.12707164595020717 No Hit GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA 981 0.11240512594874051 No Hit CTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCC 955 0.10942598907344261 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 932 0.10679059876067906 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.001031239687603124 0.0 2 0.0 0.0 0.0 0.001031239687603124 0.0 3 0.0 0.0 0.0 0.001031239687603124 0.0 4 0.0 0.0 0.0 0.0012604040626260403 0.0 5 0.0 0.0 0.0 0.0018333150001833315 0.0 6 0.0 0.0 0.0 0.0019478971876947897 0.0 7 0.0 0.0 0.0 0.002062479375206248 0.0 8 0.0 0.0 0.0 0.0021770615627177063 0.0 9 0.0 0.0 0.0 0.0024062259377406227 0.0 10-11 1.1458218751145822E-4 0.0 0.0 0.004354123125435412 0.0 12-13 1.1458218751145822E-4 0.0 0.0 0.00842179078209218 0.0 14-15 1.1458218751145822E-4 0.0 0.0 0.009739485938473947 0.0 16-17 1.1458218751145822E-4 0.0 0.0 0.014322773438932276 0.0 18-19 1.7187328126718734E-4 0.0 0.0 0.02366122172111612 0.0 20-21 2.2916437502291644E-4 0.0 0.0 0.04256728266050673 0.0 22-23 2.2916437502291644E-4 0.0 0.0 0.10913953360466394 0.0 24-25 2.2916437502291644E-4 0.0 0.0 0.2534557987753456 0.0 26-27 2.2916437502291644E-4 0.0 0.0 0.4355841858248084 0.0 28-29 2.2916437502291644E-4 0.0 0.0 0.6915607927254062 0.0 30-31 2.2916437502291644E-4 0.0 0.0 0.953839419939134 0.0 32-33 2.2916437502291644E-4 0.0 0.0 1.339179316540168 0.0 34-35 2.2916437502291644E-4 0.0 0.0 1.7036079639203607 0.0 36-37 2.2916437502291644E-4 0.0 0.0 2.183420874124592 0.0 38-39 2.2916437502291644E-4 0.0 0.0 2.7768420232464344 0.0 40-41 2.2916437502291644E-4 0.0 0.0 3.6764267773988926 0.0 42-43 2.2916437502291644E-4 0.0 0.0 4.315050599494005 0.0 44-45 2.2916437502291644E-4 0.0 0.0 4.824941333919995 0.0 46-47 2.2916437502291644E-4 0.0 0.0 5.328701921314121 0.0 48-49 2.2916437502291644E-4 0.0 0.0 5.807139845268214 0.0 50-51 2.2916437502291644E-4 0.0 0.0 6.284890276097239 0.0 52-53 2.2916437502291644E-4 0.0 0.0 6.777880137865289 0.0 54-55 2.2916437502291644E-4 0.0 0.0 7.237240127598724 0.0 56-57 8.020753125802076E-4 0.0 0.0 7.700896949363839 0.0 58-59 8.020753125802076E-4 0.0 0.0 8.18878790378763 0.0 60-61 8.020753125802076E-4 0.0 0.0 8.636517801488651 0.0 62-63 8.020753125802076E-4 0.0 0.0 9.085049774502256 0.0 64-65 8.020753125802076E-4 0.0 0.0 9.525389121108788 0.0 66-67 9.166575000916658E-4 0.0 0.0 9.958738954277123 0.0 68-69 9.166575000916658E-4 0.0 0.0 10.424229091042424 0.0 70-71 9.166575000916658E-4 0.0 0.0 10.91710437062296 0.0 72-73 9.166575000916658E-4 0.0 0.0 11.368386316136839 0.0 74-75 9.166575000916658E-4 0.0 0.0 11.833246250870825 0.0 76-77 9.166575000916658E-4 0.0 0.0 12.28229384372823 0.0 78-79 9.166575000916658E-4 0.0 0.0 12.741424669086642 0.0 80-81 9.166575000916658E-4 0.0 0.0 13.193222234444322 0.0 82-83 9.166575000916658E-4 0.0 0.0 13.664785227147728 0.0 84-85 9.166575000916658E-4 0.0 0.0 14.109020368129652 0.0 86-87 9.166575000916658E-4 0.0 0.0 14.543114985516812 0.0 88-89 9.166575000916658E-4 0.0 0.0 14.995771917280827 0.0 90-91 9.166575000916658E-4 0.0 0.0 15.433934202324643 0.0 92-93 9.166575000916658E-4 0.0 0.0 15.847690481428518 0.0 94-95 9.166575000916658E-4 0.0 0.0 16.29960262897371 0.0 96-97 9.166575000916658E-4 0.0 0.0 16.73530139698603 0.0 98-99 9.166575000916658E-4 0.0 0.0 17.184463572030946 0.0 100-101 9.166575000916658E-4 0.0 0.0 17.64938079785869 0.0 102-103 9.739485938473948E-4 0.0 0.0 18.103641880247864 0.0 104-105 0.0011458218751145821 0.0 0.0 18.5385385729476 0.0 106-107 0.0011458218751145821 0.0 0.0 18.989935100648992 0.0 108-109 0.0011458218751145821 0.0 0.0 19.429243207567925 0.0 110-111 0.0011458218751145821 0.0 0.0 19.875483536831297 0.0 112-113 0.0011458218751145821 0.0 0.0 20.320062424375756 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCATAG 275 0.0 64.90309 1 GTATAAC 270 0.0 63.9015 1 TATCACG 140 0.0 50.992363 2 AGATTAC 530 0.0 47.143883 2 TAACGCA 400 0.0 44.618317 4 ATAACGC 425 0.0 43.3935 3 TAGTACT 460 0.0 41.38511 5 GTGTTAT 550 0.0 38.941853 1 ATAGTAC 475 0.0 38.825764 4 TACGCAG 185 0.0 38.588818 5 GCATAGT 490 0.0 37.637222 2 GATTACT 685 0.0 37.344772 3 GTATCAC 210 0.0 36.82993 1 GAGTTCT 540 0.0 36.35775 1 ATCACGC 215 0.0 35.971355 3 GTACTTT 14185 0.0 35.60862 1 TCACGCA 220 0.0 35.153824 4 ATCTACG 170 3.274181E-10 34.99476 2 TACAACG 190 2.910383E-11 34.44221 2 ACGCATA 140 8.068491E-8 33.996857 1 >>END_MODULE