FastQCFastQC Report
Fri 10 Feb 2017
SRR3557637.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557637.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10614545
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT816820.7695289812234062No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT464700.43779549665105755No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT388820.3663086830382273No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166960.15729360043223709No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA541700.062.472721
GGTATCA248300.058.5138361
TATCAAC735200.045.9071772
ATCAACG750150.044.873313
TCAACGC765350.044.0598534
AACGCAG776350.043.8034136
CAACGCA772300.043.655655
ACGCAGA898200.037.8279157
CGCAGAG905450.037.5184568
GCAGAGT960650.035.4554989
AGAGTAC931900.030.29285410-11
GGACCGT25350.026.5200186
AGTACTT536950.026.01028312-13
CAGAGTA954750.025.42399210-11
AGTACAT573800.024.98271812-13
GAGTACT484200.024.97962212-13
GTACATG696650.023.801921
TACTTTT587800.023.67412614-15
ACATGGG691650.023.2505363
TACATGG708600.023.2233222
Produced by FastQC (version 0.11.5)