##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3557633.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1813656 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.53634978187705 33.0 32.0 34.0 27.0 34.0 2 31.83799904722836 33.0 32.0 34.0 27.0 34.0 3 31.929562717516443 33.0 32.0 34.0 28.0 34.0 4 31.822294856356443 33.0 32.0 34.0 28.0 34.0 5 31.846379908869157 33.0 32.0 34.0 28.0 34.0 6 34.98165638908371 37.0 35.0 38.0 29.0 38.0 7 35.34640913161041 38.0 36.0 38.0 29.0 38.0 8 35.68840342380253 38.0 36.0 38.0 30.0 38.0 9 35.81035212851831 38.0 36.0 38.0 31.0 38.0 10-11 35.770025296969216 38.0 36.0 38.0 31.0 38.0 12-13 35.78756693661863 38.0 36.0 38.0 31.0 38.0 14-15 35.90694514284958 38.0 37.0 38.0 31.0 38.0 16-17 35.88856376291866 38.0 37.0 38.0 31.0 38.0 18-19 35.919764277238905 38.0 37.0 38.0 31.0 38.0 20-21 35.79491921290476 38.0 36.5 38.0 31.0 38.0 22-23 35.779899550962256 38.0 36.5 38.0 31.0 38.0 24-25 35.72452714296426 38.0 36.0 38.0 30.0 38.0 26-27 35.58524521739514 38.0 36.0 38.0 29.0 38.0 28-29 35.45798982828056 38.0 36.0 38.0 29.0 38.0 30-31 35.20808163179787 38.0 36.0 38.0 28.0 38.0 32-33 34.986489995897784 38.0 35.5 38.0 27.0 38.0 34-35 34.68507864777003 38.0 35.0 38.0 25.0 38.0 36-37 34.376449833926614 38.0 35.0 38.0 24.0 38.0 38-39 34.114601666468175 38.0 34.5 38.0 16.0 38.0 40-41 33.78069738693556 38.0 34.0 38.0 15.5 38.0 42-43 33.38769287009223 38.0 33.5 38.0 15.0 38.0 44-45 32.810835682180084 38.0 32.0 38.0 15.0 38.0 46-47 32.25997736064612 38.0 28.5 38.0 15.0 38.0 48-49 31.71302192918613 38.0 26.0 38.0 15.0 38.0 50-51 31.294774477629716 38.0 25.0 38.0 15.0 38.0 52-53 30.988045417653623 37.5 24.5 38.0 15.0 38.0 54-55 30.677574744052897 37.0 24.0 38.0 15.0 38.0 56-57 30.461928557565493 37.0 20.0 38.0 15.0 38.0 58-59 30.292064757594606 37.0 16.0 38.0 15.0 38.0 60-61 30.125227165460263 37.0 16.0 38.0 15.0 38.0 62-63 29.985623789737414 37.0 15.0 38.0 15.0 38.0 64-65 29.8291448323166 36.0 15.0 38.0 15.0 38.0 66-67 29.715742952357004 36.0 15.0 38.0 14.0 38.0 68-69 29.550381660028144 36.0 15.0 38.0 14.0 38.0 70-71 29.378716250490722 36.0 15.0 38.0 14.0 38.0 72-73 29.175636394112225 36.0 15.0 38.0 14.0 38.0 74-75 28.997214741935625 36.0 15.0 38.0 14.0 38.0 76-77 28.83936700234223 36.0 14.0 38.0 14.0 38.0 78-79 28.682498500266867 35.0 14.0 38.0 14.0 38.0 80-81 28.534493862121593 35.0 14.0 38.0 14.0 38.0 82-83 28.35267217156947 35.0 14.0 38.0 13.0 38.0 84-85 28.218716228435824 35.0 14.0 38.0 13.0 38.0 86-87 28.098743366989112 34.0 14.0 38.0 13.0 38.0 88-89 27.974632730793488 34.0 14.0 38.0 13.0 38.0 90-91 27.7957244924065 34.0 14.0 38.0 13.0 38.0 92-93 27.723077860410136 34.0 14.0 38.0 13.0 38.0 94-95 27.617244946119882 34.0 14.0 38.0 13.0 38.0 96-97 27.522110587674838 34.0 14.0 38.0 13.0 38.0 98-99 27.38065846003873 34.0 14.0 38.0 13.0 38.0 100-101 27.249736168270058 33.0 14.0 38.0 13.0 38.0 102-103 27.107224027048126 33.0 14.0 38.0 13.0 38.0 104-105 26.983498524527253 32.5 14.0 38.0 13.0 38.0 106-107 26.860484568187132 31.5 14.0 38.0 13.0 38.0 108-109 26.64035765327052 31.0 14.0 38.0 13.0 38.0 110-111 26.536701006144494 30.0 14.0 38.0 13.0 38.0 112-113 26.408413999126626 29.0 14.0 38.0 13.0 38.0 114-115 26.314269629962904 28.5 14.0 38.0 13.0 38.0 116-117 26.1008002620122 27.0 14.0 38.0 13.0 38.0 118-119 25.92184901657205 25.0 14.0 38.0 13.0 38.0 120-121 25.806638634889968 24.5 14.0 38.0 13.0 38.0 122-123 25.62278100146886 24.0 14.0 38.0 13.0 38.0 124-125 24.531142620210225 23.5 14.0 36.0 13.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.12112589652101136 1101 2 -0.040550287923100115 1101 3 0.09354643710428334 1101 4 0.09777803387347461 1101 5 0.22754208409713428 1101 6 0.0967870119957297 1101 7 0.1997291986981935 1101 8 0.2626821379076816 1101 9 0.10474657414013677 1101 10-11 0.10717248520983702 1101 12-13 0.12470497467051445 1101 14-15 0.05586208659617142 1101 16-17 -0.19366162183675328 1101 18-19 0.007201084897467069 1101 20-21 0.07617829207718785 1101 22-23 0.020970470823108656 1101 24-25 0.0029121284067556985 1101 26-27 0.07441349551133669 1101 28-29 -0.26431748941751465 1101 30-31 -0.016757258992939228 1101 32-33 -0.05222497649265989 1101 34-35 -0.45004659736348884 1101 36-37 -0.34183765134617516 1101 38-39 -0.2702892902631291 1101 40-41 -0.11164777188715647 1101 42-43 -0.2551773079029189 1101 44-45 -0.11731179880152354 1101 46-47 -0.043958776700016244 1101 48-49 -0.3623669650006427 1101 50-51 -0.031022703499854032 1101 52-53 0.06468936490956523 1101 54-55 0.08526774510796997 1101 56-57 0.10543187025547951 1101 58-59 0.07243781791732218 1101 60-61 0.08142856869637072 1101 62-63 0.22072577090744616 1101 64-65 0.19297251988315267 1101 66-67 0.16687048201883314 1101 68-69 0.15571641788956825 1101 70-71 0.08798135721025346 1101 72-73 -0.0819890199698321 1101 74-75 -0.059720836518927456 1101 76-77 -0.1642239065691058 1101 78-79 -0.19976774915541995 1101 80-81 -0.2995299635604489 1101 82-83 -0.22171845791033817 1101 84-85 -0.09630714626490544 1101 86-87 0.0218652777803392 1101 88-89 -6.334460125465569E-4 1101 90-91 -0.017924225450304476 1101 92-93 -0.1588891338459213 1101 94-95 -0.14682993416984047 1101 96-97 -0.1384176526580987 1101 98-99 0.0654125653057065 1101 100-101 -0.31190975303536206 1101 102-103 -0.2215863284598818 1101 104-105 -0.31493198263412836 1101 106-107 -0.2548690389046797 1101 108-109 -0.3569889921045082 1101 110-111 -0.44919695230434087 1101 112-113 -0.31559896278769983 1101 114-115 -0.13768253662344776 1101 116-117 -0.2812896062260606 1101 118-119 -0.37520255865404906 1101 120-121 -0.1844708507496371 1101 122-123 -0.5298905733359618 1101 124-125 -0.3477285803538166 1102 1 -0.04923541085641503 1102 2 0.046191919612201815 1102 3 -0.07229280942251748 1102 4 0.02231771559992879 1102 5 0.19584736711922446 1102 6 0.22840060935208584 1102 7 0.019672386500083405 1102 8 0.12505771890918282 1102 9 0.10329325207816709 1102 10-11 0.17057501215720805 1102 12-13 0.23154777053654385 1102 14-15 0.07282519566933132 1102 16-17 0.006666841472984686 1102 18-19 0.015717079694425706 1102 20-21 0.17229138136853805 1102 22-23 0.16938408411875372 1102 24-25 -0.11517142764974864 1102 26-27 -0.14306963303053521 1102 28-29 0.030869413036853643 1102 30-31 0.01771900711357688 1102 32-33 0.04441183246066771 1102 34-35 -0.1145143530254984 1102 36-37 -0.32828654740774965 1102 38-39 -0.02756187780827446 1102 40-41 -0.2673214489969027 1102 42-43 -0.16699828686020624 1102 44-45 -0.15307729763027567 1102 46-47 -0.11812872373032945 1102 48-49 -0.08602329674289422 1102 50-51 0.06215897883570776 1102 52-53 -0.011303354248607178 1102 54-55 0.13025455249583473 1102 56-57 -0.006337163680985469 1102 58-59 -0.07190600973336458 1102 60-61 0.10323409223102331 1102 62-63 0.1598405139005621 1102 64-65 0.207177134878048 1102 66-67 0.11524382013395851 1102 68-69 0.2621193177025667 1102 70-71 0.2351902729682358 1102 72-73 0.040497339125384 1102 74-75 0.11322761978890483 1102 76-77 0.0572160094080445 1102 78-79 -0.02796549886865307 1102 80-81 -0.28127320999616856 1102 82-83 0.07454406226283083 1102 84-85 -0.2442667743100948 1102 86-87 -0.04761808985093907 1102 88-89 -0.08210344740723485 1102 90-91 -0.16581340750835238 1102 92-93 -0.06579340984618298 1102 94-95 -0.227177069224048 1102 96-97 0.07654564427102173 1102 98-99 0.09157165084563701 1102 100-101 -0.08715745251135942 1102 102-103 -0.013744480412587023 1102 104-105 0.1599215039441333 1102 106-107 0.01349971666340366 1102 108-109 -0.11457396135021014 1102 110-111 -0.2508974757393716 1102 112-113 0.10966851849074644 1102 114-115 -0.025441158785326934 1102 116-117 -0.024247996129656002 1102 118-119 -0.0051449494912283456 1102 120-121 0.08211640407698084 1102 122-123 -0.08073635212702612 1102 124-125 -0.04160575670787381 1103 1 0.23116941619944598 1103 2 0.2180105436132429 1103 3 0.2631810512711077 1103 4 0.31177965810676866 1103 5 0.35819176630511507 1103 6 -0.022892429676851123 1103 7 0.2710377063699738 1103 8 0.3100654225251276 1103 9 0.44157112028247525 1103 10-11 0.381217414924528 1103 12-13 0.3351004010428653 1103 14-15 0.2268734662279286 1103 16-17 0.3345249916812705 1103 18-19 0.25684474233310794 1103 20-21 0.3832017123658815 1103 22-23 0.2917430481658272 1103 24-25 0.35264444098389447 1103 26-27 0.32028792072494383 1103 28-29 0.3248773041482096 1103 30-31 0.05234125819982438 1103 32-33 0.13587195979451394 1103 34-35 0.003519716793448424 1103 36-37 0.18793153421525943 1103 38-39 0.22475885347818547 1103 40-41 0.24239203627112005 1103 42-43 0.17636464719847567 1103 44-45 -0.16834988607240575 1103 46-47 0.13075323296094865 1103 48-49 0.24166923251590333 1103 50-51 0.04713380282099067 1103 52-53 0.08185741084137632 1103 54-55 0.2652368491244488 1103 56-57 0.07125659081958347 1103 58-59 0.27654878349180123 1103 60-61 0.12373834620307278 1103 62-63 0.1945869467323682 1103 64-65 0.1772515080591397 1103 66-67 0.08824570324007297 1103 68-69 -0.038535829318295356 1103 70-71 -0.016844459877969342 1103 72-73 0.14013960330914088 1103 74-75 0.08575886223134788 1103 76-77 0.009256041037701834 1103 78-79 0.1166616705755743 1103 80-81 0.012758050108882202 1103 82-83 -0.08381388550474966 1103 84-85 -0.10184892583356486 1103 86-87 0.0891262560753816 1103 88-89 0.14739175515574487 1103 90-91 0.01013962624897502 1103 92-93 -0.03882002060841927 1103 94-95 0.06989800230952525 1103 96-97 0.14670805877719317 1103 98-99 0.14582738915638416 1103 100-101 0.08629292506093122 1103 102-103 0.20224385139451684 1103 104-105 0.20408998895401353 1103 106-107 0.3016125073831937 1103 108-109 0.17319902789733632 1103 110-111 0.14026225133251913 1103 112-113 0.05762835099483965 1103 114-115 -0.031105558297294778 1103 116-117 0.06261985221101085 1103 118-119 0.3829899874260754 1103 120-121 0.5289072434314512 1103 122-123 0.27977833637243776 1103 124-125 0.06196399294498889 1104 1 0.05019451813944542 1104 2 0.1917013367776086 1104 3 0.20038695332498335 1104 4 0.37121779497011786 1104 5 0.3215708156465844 1104 6 0.21089921501096853 1104 7 0.3592984885658552 1104 8 0.3961591947656302 1104 9 0.33483083493420196 1104 10-11 0.33664090550083614 1104 12-13 0.24432932018581965 1104 14-15 0.2446143230263047 1104 16-17 0.2884913368287627 1104 18-19 0.12870255173252332 1104 20-21 0.28219790688498847 1104 22-23 0.20222162192684578 1104 24-25 0.2880339480715506 1104 26-27 0.2688924730680924 1104 28-29 0.13314218906276665 1104 30-31 0.10949333300327169 1104 32-33 0.07789907960227538 1104 34-35 0.1574294925673101 1104 36-37 0.22859345345030846 1104 38-39 0.3981484317354571 1104 40-41 0.44217455031665764 1104 42-43 0.4146721606160426 1104 44-45 0.547459657772265 1104 46-47 0.3803306634031216 1104 48-49 0.5333241247986038 1104 50-51 0.4066326955055892 1104 52-53 0.18750555666371937 1104 54-55 0.1604709971404894 1104 56-57 0.28276351311074066 1104 58-59 0.06175968625736772 1104 60-61 0.27041760361099065 1104 62-63 0.20186246941035435 1104 64-65 0.15920818041937324 1104 66-67 0.112774361567336 1104 68-69 0.23188906371796847 1104 70-71 0.09490360179677637 1104 72-73 0.13770570731724519 1104 74-75 0.23062041640816489 1104 76-77 -0.011442017706528418 1104 78-79 0.2878504856434354 1104 80-81 0.23229641052853367 1104 82-83 0.3451203809877619 1104 84-85 0.04906492335384627 1104 86-87 0.22018483075742168 1104 88-89 0.22446242448499376 1104 90-91 -0.03570877829583097 1104 92-93 0.16579941036961898 1104 94-95 0.01507280743038919 1104 96-97 0.06664407939125994 1104 98-99 0.09865973030162678 1104 100-101 0.2101782833789798 1104 102-103 0.1601690060837555 1104 104-105 0.12189379945864687 1104 106-107 -0.10150419821743384 1104 108-109 0.09223417731164929 1104 110-111 -0.11061951919991486 1104 112-113 -0.2023722874384255 1104 114-115 -0.23144486987784418 1104 116-117 -0.10115502575403923 1104 118-119 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0.27296703801368594 1105 116-117 0.3602331734572921 1105 118-119 0.42608967887736426 1105 120-121 0.4574273916006142 1105 122-123 0.44544031827886243 1105 124-125 0.10782875158425043 1106 1 0.10609991172486488 1106 2 0.006686815896159715 1106 3 0.10244330563118709 1106 4 0.008989318322431217 1106 5 0.15157774858651507 1106 6 0.20024259103024633 1106 7 0.18023818408080672 1106 8 0.30269424642006015 1106 9 0.3099425378980385 1106 10-11 0.23604765040492026 1106 12-13 0.2624141660689787 1106 14-15 0.07843424696550017 1106 16-17 0.12099256621635845 1106 18-19 0.0706430846418229 1106 20-21 0.20179822491345334 1106 22-23 0.1413223450486072 1106 24-25 0.25979884482314475 1106 26-27 0.12974469060117855 1106 28-29 0.1482707104521026 1106 30-31 0.20609485724236265 1106 32-33 0.1445616147204305 1106 34-35 0.19921368163503672 1106 36-37 0.18405485672571587 1106 38-39 -0.09467401043294643 1106 40-41 -0.10686239580392254 1106 42-43 -0.21699966040930718 1106 44-45 -0.23279272441813958 1106 46-47 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100-101 0.34885831162161907 2201 102-103 0.08818382521112156 2201 104-105 0.15868277863589242 2201 106-107 0.13758919862054597 2201 108-109 0.18379307601389527 2201 110-111 0.0824262987011295 2201 112-113 -0.0018589702496392135 2201 114-115 -0.027310771020349733 2201 116-117 0.2249075828913476 2201 118-119 0.35761717746069266 2201 120-121 0.20618216747294227 2201 122-123 0.15954486692661618 2201 124-125 -0.17898728115473972 2202 1 0.23658340370885256 2202 2 0.29005525423398737 2202 3 0.2546388643521773 2202 4 0.33945182260681506 2202 5 0.3951846985272063 2202 6 0.5057717567667623 2202 7 0.31892193952843684 2202 8 0.336745817376098 2202 9 0.4121281007412563 2202 10-11 0.2189019872677065 2202 12-13 0.39093443365243274 2202 14-15 0.3428702741980132 2202 16-17 0.24883304123796535 2202 18-19 0.43399773522698837 2202 20-21 0.5603815746231788 2202 22-23 0.5879282040661664 2202 24-25 0.49899631308471015 2202 26-27 0.3505525372802083 2202 28-29 0.3576923657257325 2202 30-31 0.46662824116641133 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0.3357213058510311 2204 30-31 0.3840289326066042 2204 32-33 0.3982846878629971 2204 34-35 0.3837399566037121 2204 36-37 0.33475574813230224 2204 38-39 0.3608562092246572 2204 40-41 0.36481973877241103 2204 42-43 0.34779697861397807 2204 44-45 0.55397557754182 2204 46-47 0.41172872454007603 2204 48-49 0.1970480148811653 2204 50-51 0.1407043177440812 2204 52-53 0.25190969175056566 2204 54-55 0.2210373482057406 2204 56-57 0.17231506986783884 2204 58-59 -0.03092781521832677 2204 60-61 0.03276791921240374 2204 62-63 0.09146051047789072 2204 64-65 0.11008065895296681 2204 66-67 0.16274450339121316 2204 68-69 0.019218424487373653 2204 70-71 -0.2288650312791951 2204 72-73 -0.005197884714799983 2204 74-75 -0.004384581270077348 2204 76-77 0.03284326511483471 2204 78-79 0.17330306082504165 2204 80-81 0.050686882671396205 2204 82-83 -0.04433427196874007 2204 84-85 -0.12583045005535354 2204 86-87 -0.09211422912814626 2204 88-89 0.05519780135715635 2204 90-91 -0.05350021770587077 2204 92-93 -0.23952951162725 2204 94-95 0.06259846480216069 2204 96-97 0.08106331573028314 2204 98-99 -0.06190248882492 2204 100-101 0.053099682099258416 2204 102-103 -0.1713433228343071 2204 104-105 -0.3464629285181182 2204 106-107 -0.3229756209423087 2204 108-109 -0.4414598152429896 2204 110-111 -0.4012743527811651 2204 112-113 -0.2730641018710891 2204 114-115 -0.23598311588954246 2204 116-117 -0.11800251392882544 2204 118-119 0.036302457579399317 2204 120-121 -0.21164999090446202 2204 122-123 -0.2720255244789911 2204 124-125 -0.22272818874612454 2205 1 0.35973054680929195 2205 2 0.333650845609359 2205 3 0.36667451580830956 2205 4 0.3780010778528684 2205 5 0.5292793156987763 2205 6 0.5501061261599602 2205 7 0.33867464110056034 2205 8 0.3860857514848348 2205 9 0.4684110571486926 2205 10-11 0.48845473417764396 2205 12-13 0.2845382841513455 2205 14-15 0.2800786857833373 2205 16-17 0.3402097008383649 2205 18-19 0.3764797544510543 2205 20-21 -0.037092624643726424 2205 22-23 0.12778890095912487 2205 24-25 0.2379327569740468 2205 26-27 0.3470437494096785 2205 28-29 0.35182803854608125 2205 30-31 0.4259471069383025 2205 32-33 0.48297516139019336 2205 34-35 0.5517750413188054 2205 36-37 0.4713114637259892 2205 38-39 0.4105399919919961 2205 40-41 0.2851732281636288 2205 42-43 0.3849907676002502 2205 44-45 0.33385982072182685 2205 46-47 0.26946733517283405 2205 48-49 0.29651339854226677 2205 50-51 0.38233269724526764 2205 52-53 0.3173708478273305 2205 54-55 0.48766907653925884 2205 56-57 0.4109735759710027 2205 58-59 0.35122240212204403 2205 60-61 0.4209150781827411 2205 62-63 0.5111353722321077 2205 64-65 0.3007108102281215 2205 66-67 0.3763296919316268 2205 68-69 0.17630535925396273 2205 70-71 0.2858028543164046 2205 72-73 0.34121361703970976 2205 74-75 0.14650712877029548 2205 76-77 0.06275765929380839 2205 78-79 0.10887670544557437 2205 80-81 0.22137560053583982 2205 82-83 0.18020389988642904 2205 84-85 0.23319901460560288 2205 86-87 0.2290481125963737 2205 88-89 0.22228819313140846 2205 90-91 0.24134824245269115 2205 92-93 0.2571446720747339 2205 94-95 0.2695531954939021 2205 96-97 0.436301464891649 2205 98-99 0.08975404388385044 2205 100-101 0.3883673976524342 2205 102-103 0.3766498174681878 2205 104-105 0.17708281515051638 2205 106-107 0.17577173163886783 2205 108-109 -0.39505604687446905 2205 110-111 -0.4316018846940395 2205 112-113 -0.30154137515284063 2205 114-115 -0.1451845333087185 2205 116-117 -0.06343162248577272 2205 118-119 -0.11747511735043759 2205 120-121 -0.046641859341153946 2205 122-123 -0.15058731262195835 2205 124-125 0.054351896450011594 2206 1 0.33481133082437253 2206 2 0.4342400248833762 2206 3 0.4297147402729884 2206 4 0.25882172957548377 2206 5 0.45248032280128925 2206 6 0.3797693818834844 2206 7 0.3507134228287754 2206 8 0.38551347277362424 2206 9 0.3673220622955995 2206 10-11 0.34703809344691905 2206 12-13 0.29281618431379997 2206 14-15 0.23217571706783247 2206 16-17 0.2722891294328136 2206 18-19 0.30747572419567604 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86-87 0.25698602564466455 2206 88-89 0.31914788402889016 2206 90-91 0.37003128803762664 2206 92-93 0.28841555163988275 2206 94-95 0.28020392567247754 2206 96-97 0.294997569593221 2206 98-99 0.2890572501073976 2206 100-101 0.34143311698742096 2206 102-103 0.2658817060171934 2206 104-105 0.22243213358074598 2206 106-107 0.26638991641940635 2206 108-109 0.6348387083025351 2206 110-111 0.5701879771736813 2206 112-113 0.24180879348147144 2206 114-115 0.3086904326819955 2206 116-117 0.3321176109240511 2206 118-119 0.46546006257754513 2206 120-121 0.3924554660716417 2206 122-123 0.4895031627744437 2206 124-125 0.3445435614344987 2207 1 0.19381240431830804 2207 2 0.3281444262085067 2207 3 0.3568300427558775 2207 4 -0.558253749745333 2207 5 0.1967740676791081 2207 6 0.45600084102722604 2207 7 0.4766155617365868 2207 8 0.39101691834286356 2207 9 0.4219649812756643 2207 10-11 0.5144356209479923 2207 12-13 0.4297561494541142 2207 14-15 0.4723277376604429 2207 16-17 0.4522514994303819 2207 18-19 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-0.18393278938074786 2314 36-37 -0.05718285091467834 2314 38-39 -0.08278929026312909 2314 40-41 -0.030842755705926095 2314 42-43 -0.07913901232578979 2314 44-45 0.04503987325888659 2314 46-47 0.1416871240551032 2314 48-49 0.24105806196807222 2314 50-51 0.1196649987655185 2314 52-53 0.07200462920298634 2314 54-55 0.13957599753515382 2314 56-57 0.19709179474307348 2314 58-59 0.3403383033542191 2314 60-61 0.3341928621160015 2314 62-63 0.08119637500669441 2314 64-65 0.259045874791461 2314 66-67 0.3410209674557265 2314 68-69 -1.4064801840521746 2314 70-71 -0.5242085025524226 2314 72-73 0.2123070965350209 2314 74-75 0.36213730803339317 2314 76-77 0.19014502546972523 2314 78-79 0.350663750305209 2314 80-81 0.413590316914199 2314 82-83 0.28920387218675003 2314 84-85 0.4325156692690726 2314 86-87 0.28396748921507253 2314 88-89 0.5514835982161479 2314 90-91 0.6352917939671734 2314 92-93 0.6602519125402715 2314 94-95 0.41610830746985883 2314 96-97 0.6735092621207563 2314 98-99 0.5381404509583483 2314 100-101 0.5305592329409023 2314 102-103 0.5639449552510136 2314 104-105 0.661335547031463 2314 106-107 0.8084063116886355 2314 108-109 0.5485867899882635 2314 110-111 0.4565473842652352 2314 112-113 0.4906308106750821 2314 114-115 0.44949383878108407 2314 116-117 0.45343935817523473 2314 118-119 0.4711094693934186 2314 120-121 0.3256424178587771 2314 122-123 0.4357243133954256 2314 124-125 0.41996963971091006 2315 1 -0.2997985720736871 2315 2 -0.253164104901181 2315 3 -0.39849372638945013 2315 4 -1.0060063741606093 2315 5 -0.3801310778957614 2315 6 -0.5257554442713541 2315 7 -0.8293973608685903 2315 8 -0.5046250660436229 2315 9 -0.4408276156047606 2315 10-11 -0.6723234021906705 2315 12-13 -0.4145133165888524 2315 14-15 -0.42100397904351183 2315 16-17 -0.35884128988109154 2315 18-19 -0.27963309572754014 2315 20-21 -0.08272781942487484 2315 22-23 -0.3571631071834318 2315 24-25 -0.3924764113938153 2315 26-27 -0.44071493370192627 2315 28-29 -0.3372904418190288 2315 30-31 0.008562369596461394 2315 32-33 -0.16646162170198409 2315 34-35 -0.0927962162725251 2315 36-37 -0.12258994533043932 2315 38-39 0.08183305602556601 2315 40-41 0.08769792104106244 2315 42-43 -7.424331145671204E-4 2315 44-45 0.16289921799692308 2315 46-47 0.13970890643988554 2315 48-49 0.038022290967248296 2315 50-51 -0.12833946326827217 2315 52-53 -0.4313082284914529 2315 54-55 -0.3821793363545041 2315 56-57 -0.26630135153565604 2315 58-59 -0.2909626068010347 2315 60-61 -0.3441308548546722 2315 62-63 -0.2699830825121303 2315 64-65 -0.08708090550708647 2315 66-67 -0.30905851154811614 2315 68-69 -0.21188759762946674 2315 70-71 -0.04473292385808847 2315 72-73 -0.11779115850773181 2315 74-75 -0.11385201484597474 2315 76-77 -0.3439896887172367 2315 78-79 -0.07658454801896042 2315 80-81 -0.025980340776502686 2315 82-83 -0.12404257655746775 2315 84-85 -0.1682477106047564 2315 86-87 -0.36722185452655864 2315 88-89 -0.08569786301429261 2315 90-91 -0.19415899132586745 2315 92-93 -0.1131568337309865 2315 94-95 -0.1617307121282181 2315 96-97 -0.08634952058162426 2315 98-99 -0.43343101475591794 2315 100-101 -0.23237400936652364 2315 102-103 -0.1386264746548882 2315 104-105 -0.43707829803975073 2315 106-107 -0.21948587554864218 2315 108-109 -0.23605566347643148 2315 110-111 -0.329574694239156 2315 112-113 -0.28100289153631763 2315 114-115 -0.1688897871429269 2315 116-117 -0.07201202184805311 2315 118-119 -0.06004517137082743 2315 120-121 -0.16210567184273472 2315 122-123 -0.17099006662163418 2315 124-125 0.044974504978039676 2316 1 -0.41466337623781513 2316 2 -0.49602924325927944 2316 3 -0.2735703125081841 2316 4 -0.462858518482097 2316 5 -0.40549074068326263 2316 6 -0.7011515809171627 2316 7 -0.6921806396708092 2316 8 -0.8463213340312521 2316 9 -0.783990474077207 2316 10-11 -0.8930915362563567 2316 12-13 -0.854576705639154 2316 14-15 -0.58509591472734 2316 16-17 -0.5063650203482624 2316 18-19 -0.5161166564385482 2316 20-21 -0.49030072671479275 2316 22-23 -0.5378847864901743 2316 24-25 -0.5845492315417644 2316 26-27 -0.48209389708971884 2316 28-29 -0.39913047610085783 2316 30-31 -0.2073898226980333 2316 32-33 -0.26138663468344703 2316 34-35 -0.2828609528791546 2316 36-37 -0.615414881591029 2316 38-39 -0.21056151565813508 2316 40-41 -0.4197336275886556 2316 42-43 -0.30018786876156156 2316 44-45 -0.25905166626954923 2316 46-47 -0.08249684564928117 2316 48-49 0.10303829095287043 2316 50-51 0.007411116630141379 2316 52-53 0.052752875103291075 2316 54-55 0.1843573809623109 2316 56-57 0.25650087390872045 2316 58-59 0.29217959812455163 2316 60-61 0.2564226763718018 2316 62-63 0.05893273654159614 2316 64-65 0.12116887933308362 2316 66-67 0.038875594743533526 2316 68-69 -0.07128311978958024 2316 70-71 0.08030660446125992 2316 72-73 0.3772655556468223 2316 74-75 0.3759712396642989 2316 76-77 0.3739447589553535 2316 78-79 0.5721834261367036 2316 80-81 0.5200175965639211 2316 82-83 0.5490961786238877 2316 84-85 0.37158874995765956 2316 86-87 0.360550428215916 2316 88-89 0.2082086668843921 2316 90-91 0.31645952805109445 2316 92-93 0.3798075746109255 2316 94-95 0.2954026222831594 2316 96-97 0.08060911662566639 2316 98-99 -0.09012553990401528 2316 100-101 0.13459927128634064 2316 102-103 0.3677764393009433 2316 104-105 0.302741138394218 2316 106-107 0.15763787324529588 2316 108-109 0.37275614219516484 2316 110-111 0.1261477486828504 2316 112-113 0.03587026568038354 2316 114-115 -0.05996164244053048 2316 116-117 -0.2677021324015776 2316 118-119 -0.29628280304953947 2316 120-121 -0.15473274191387176 2316 122-123 -0.04904969329462716 2316 124-125 -0.17345741557722505 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 11.0 15 157.0 16 401.0 17 663.0 18 2521.0 19 11746.0 20 38906.0 21 86305.0 22 120761.0 23 138598.0 24 123455.0 25 73004.0 26 50438.0 27 47241.0 28 41447.0 29 42177.0 30 48074.0 31 55875.0 32 67691.0 33 84541.0 34 106994.0 35 139337.0 36 205235.0 37 328078.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 54.09260890997937 15.506114486298236 10.076743998826618 20.32453260489578 2 16.142917951364534 25.61908101646619 37.42970000926306 20.808301022906218 3 23.090703177897414 34.48610869119324 24.955890104606617 17.46729802630273 4 13.214468454877881 19.24444326818316 34.56807685691223 32.97301142002673 5 14.206001579130772 39.161616094783135 29.941620682202135 16.690761643883956 6 24.50569457493593 31.856316743638263 25.16375762548135 18.474231055944458 7 25.918421133886472 33.36823521108744 22.333507567035866 18.379836087990224 8 27.71407587767471 25.539297419135714 27.195013828421704 19.551612874767873 9 33.270862831760816 12.448281261716666 22.406068184925918 31.874787721596597 10-11 25.21776455954161 25.02754105519459 27.923790399061343 21.830903986202454 12-13 26.505936076080577 23.935961395104695 31.412186213923697 18.14591631489103 14-15 25.46227068418708 21.87622680375992 33.5976061612566 19.0638963507964 16-17 18.835435165213248 25.574557688999455 33.10920042168967 22.480806724097626 18-19 19.7150396767634 23.500184158407105 37.990556092224764 18.794220072604727 20-21 23.055805511078177 22.106011283286357 36.94926160197965 17.88892160365582 22-23 21.032916529393844 25.756801724743983 34.27581291931391 18.934468826548265 24-25 19.94149894480307 26.492955474962677 34.957854152623796 18.607691427610458 26-27 21.40963192049763 25.97623740646784 35.095993588020384 17.51813708501415 28-29 20.49839332461184 27.14146876736085 34.43780146703042 17.922336440996897 30-31 21.698119189931294 27.183477479052254 34.32913307716209 16.789270253854355 32-33 17.949065054928816 27.538986142487932 35.31010532503202 19.20184347755123 34-35 16.844824838700962 29.610538847138525 34.22335222989958 19.32128408426093 36-37 19.38649339292306 29.051454174474472 32.21073723405106 19.351315198551408 38-39 18.861459946097828 28.438358762631943 32.956911343716776 19.74326994755345 40-41 17.639910423805237 29.724784502425077 32.87379080269831 19.76151427107137 42-43 18.159292103709884 29.094527599094754 32.24159715006516 20.5045831471302 44-45 19.514312526741566 28.734776605927475 31.186509459346208 20.564401407984757 46-47 18.22363805036605 27.930784934571555 32.05374878178893 21.791828233273463 48-49 18.500057484590133 28.40353575720715 30.942038715113263 22.154368043089455 50-51 16.02667601893393 28.102184548466358 32.1493561493363 23.721783283263413 52-53 17.27239213698865 26.133513917269802 32.140733046887945 24.453360898853603 54-55 17.784864139014005 25.80940997081357 31.02268324930343 25.383042640869004 56-57 19.072716830168577 24.932619051623664 30.805122027414498 25.18954209079326 58-59 17.39143741595504 25.527361882265225 32.02792339979911 25.053277301980625 60-61 18.861174638381698 24.467092444908666 30.804519036213946 25.867213880495694 62-63 17.613853797332247 24.54913399981252 32.56651465941737 25.270497543437862 64-65 17.01012766548709 25.431230791455445 31.73155069953012 25.827090843527344 66-67 17.220906279911958 25.30973348859982 31.684371236882846 25.784988994605374 68-69 17.778371422144 25.480383270035773 31.622589950905795 25.11865535691443 70-71 16.92449310246626 25.811820381544344 30.744151797295572 26.519534718693823 72-73 18.064533737379087 25.36726920650884 30.637507884626412 25.93068917148566 74-75 18.049839660883872 24.60458322857256 31.02118042230721 26.324396688236355 76-77 17.62707967417186 24.624797818549332 29.86474002367043 27.883382483608383 78-79 18.54508241915777 24.266702175054146 29.6137470391298 27.574468366658284 80-81 18.34372119078811 24.249499353791457 28.432955312363532 28.9738241430569 82-83 17.965865632733 23.82968435028473 27.65328706215512 30.551162954827156 84-85 19.550939209947664 23.09472820585206 26.994787313111885 30.35954527108839 86-87 19.52573474172903 23.071434774398003 26.331550094491902 31.071280389381066 88-89 19.687171575368527 23.245847168752228 25.826623775489608 31.240357480389637 90-91 20.955932732395578 23.10744724056746 25.001826635348717 30.934793391688242 92-93 20.771006655746085 24.154769326382468 23.688103339839543 31.386120678031904 94-95 20.996944280687202 24.20999922242118 24.177158925529458 30.61589757136216 96-97 21.030004811689235 24.28858002776717 24.052352281829876 30.629062878713718 98-99 22.252600213592665 24.576227020382298 23.585803394926664 29.58536937109837 100-101 21.90598676886771 23.797976366399396 24.395723968942605 29.900312895790286 102-103 21.26559932541371 26.011665732345534 23.699751745406545 29.022983196834208 104-105 21.601692405844346 26.161185500186658 24.471481594011184 27.765640499957815 106-107 20.745980703849636 25.884057683337137 25.467737008622503 27.902224604190724 108-109 20.742382818261444 26.570640971104346 25.443271332092554 27.243704878541656 110-111 20.947872028371485 26.78341820812372 26.17991213308838 26.088797630416423 112-113 19.585885502904908 27.849346043592682 26.081670529037254 26.483097924465156 114-115 19.158301882110987 26.841709145344623 26.976216535118308 27.02377243742608 116-117 19.029904237628305 27.17083614533296 27.60118236313832 26.198077253900408 118-119 18.902647470082528 28.571515215674857 27.352044709691363 25.17379260455125 120-121 18.934241112978427 28.592550075648305 27.99315305658846 24.48005575478481 122-123 18.545633791634135 29.440505807054922 27.556796878790685 24.457063522520258 124-125 17.24360325329561 30.986002133995967 28.130899798186213 23.63949481452221 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 26.0 1 14.5 2 27.5 3 68.0 4 109.5 5 151.0 6 246.5 7 392.5 8 609.0 9 906.0 10 1238.5 11 1609.5 12 2065.0 13 2613.5 14 3251.0 15 4034.5 16 4950.0 17 5966.5 18 7130.0 19 8435.5 20 9760.0 21 11336.5 22 12946.5 23 14565.5 24 16515.0 25 18582.5 26 20915.5 27 23639.5 28 26958.0 29 30741.0 30 34487.0 31 38414.5 32 42121.5 33 44064.5 34 44024.5 35 42965.5 36 40670.0 37 37869.5 38 34084.5 39 29287.0 40 24849.0 41 21109.5 42 19209.0 43 20372.5 44 24326.0 45 28913.0 46 37970.5 47 43626.5 48 50589.5 49 65220.0 50 85411.5 51 97923.5 52 105634.5 53 116873.5 54 115024.5 55 99783.0 56 77869.0 57 54954.5 58 36897.0 59 25867.0 60 16839.0 61 11499.5 62 8003.5 63 4122.0 64 1411.5 65 346.0 66 197.5 67 158.5 68 124.5 69 118.5 70 118.5 71 98.0 72 99.0 73 91.5 74 58.5 75 40.0 76 35.5 77 23.0 78 15.0 79 15.5 80 11.5 81 9.0 82 10.0 83 5.5 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.005182901277860852 2 0.0 3 2.2054899054727028E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.00294984274856974 24-25 7.44352843097037E-4 26-27 0.009676586960261482 28-29 0.007085136321331057 30-31 0.008573842007525131 32-33 0.0070024304498758315 34-35 0.005458587516044939 36-37 0.0017919605481965708 38-39 0.0 40-41 2.4811761436567905E-4 42-43 2.7568623818408785E-5 44-45 0.0 46-47 0.0018470977958333884 48-49 0.006974861826057422 50-51 0.010586351546268974 52-53 0.015410860714490509 54-55 0.017285527134142307 56-57 0.015024899981032786 58-59 0.014694076495211882 60-61 0.019242899425249333 62-63 0.008050038154975365 64-65 5.513724763681757E-4 66-67 0.0 68-69 0.0 70-71 2.7568623818408785E-5 72-73 0.0 74-75 0.0 76-77 2.7568623818408785E-5 78-79 0.0 80-81 0.0 82-83 0.0 84-85 2.2054899054727028E-4 86-87 4.686666049129493E-4 88-89 0.0040801563251245 90-91 0.011716665122823733 92-93 0.014363253009390975 94-95 0.018470977958333884 96-97 0.020042389515983184 98-99 0.02158623244981408 100-101 0.023846859602923598 102-103 0.021861918687998166 104-105 0.007884626412064912 106-107 0.001654117429104527 108-109 5.238038525497669E-4 110-111 2.2054899054727028E-4 112-113 1.654117429104527E-4 114-115 5.513724763681757E-5 116-117 0.0 118-119 0.0 120-121 0.0 122-123 0.0 124-125 0.06032014891467842 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1813656.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 24.953819456892614 #Duplication Level Percentage of deduplicated Percentage of total 1 77.96457299458659 19.455138785406394 2 9.476171015026807 4.729333213032356 3 4.04514242694415 3.028252613981422 4 2.0079887257800935 2.004279525383692 5 1.2253111496108309 1.5288096602953105 6 0.7745967590524175 1.1597488606372905 7 0.5664252162424834 0.9894130807362402 8 0.43521643280663247 0.8688249831143633 9 0.34270089753680366 0.7696526692363608 >10 2.449257067442155 12.220383256679067 >50 0.3282696966289233 5.722831758539551 >100 0.30129873196123497 15.699069124924028 >500 0.046235659700189474 8.111016182328012 >1k 0.03396458413994961 17.263365780075542 >5k 0.002191263492899975 3.719524412785238 >10k+ 6.573790478699924E-4 2.730356092845145 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24472 1.3493187241681994 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13756 0.7584679784920625 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11705 0.6453814835889496 No Hit ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC 8756 0.48278174030797466 No Hit CACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCC 8423 0.46442103684491437 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 7792 0.4296294335860824 No Hit GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC 7320 0.4036046527015046 No Hit GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT 7186 0.396216261518171 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 6527 0.35988081532550825 No Hit GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5885 0.32448270234267135 No Hit GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT 5797 0.31963062455063146 No Hit GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA 5290 0.29167603999876496 No Hit CACAATATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCC 5047 0.27827768882301823 No Hit CAGTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGA 4921 0.27133039562077926 No Hit ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT 4913 0.2708892976396847 No Hit CATATTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAA 4837 0.26669886681928656 No Hit CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC 4806 0.26498961214254524 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 4789 0.2640522789327193 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4671 0.25754608371157484 No Hit CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT 4305 0.2373658510764996 No Hit AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA 4277 0.23582200814266874 No Hit CATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAA 4081 0.2250151076058525 No Hit GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT 3972 0.21900514761343937 No Hit GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA 3796 0.20930099202935948 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 3708 0.20444891423731953 No Hit CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATT 3641 0.20075471864565275 No Hit GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTG 3590 0.19794271901617508 No Hit GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT 3588 0.19783244452090143 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 3584 0.19761189553035413 No Hit TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCA 3561 0.19634373883470735 No Hit TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT 3531 0.19468962140560284 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 3454 0.19044405333756786 No Hit ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAG 3224 0.17776248638109984 No Hit GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCG 3141 0.17318609482724398 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3130 0.17257958510323898 No Hit GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC 3121 0.17208334987450763 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 3091 0.1704292324454031 No Hit GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACA 3091 0.1704292324454031 No Hit GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGT 3052 0.16827887978756723 No Hit GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2983 0.16447440970062682 No Hit GTACATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTC 2953 0.16282029227152228 No Hit ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG 2803 0.15454970512599964 No Hit CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCA 2798 0.15427401888781556 No Hit TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGG 2754 0.15184797999179558 No Hit CTGCACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT 2746 0.15140688201070104 RNA PCR Primer, Index 23 (95% over 23bp) GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2745 0.15135174476306423 No Hit GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGC 2700 0.14887056861940742 No Hit GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAA 2690 0.14831919614303926 No Hit ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGT 2679 0.14771268641903426 No Hit CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG 2642 0.145672608256472 No Hit GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA 2612 0.14401849082736748 No Hit GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAA 2571 0.14175786367425797 No Hit CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACAC 2552 0.14071025596915843 No Hit CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGCGG 2464 0.13585817817711848 No Hit CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAA 2443 0.13470029597674532 No Hit GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATA 2407 0.1327153550618199 No Hit CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTG 2390 0.131778021851994 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 2385 0.13150233561380992 No Hit TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCG 2360 0.13012390442288946 No Hit GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACACAC 2356 0.1299033554323422 No Hit GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC 2339 0.1289660222225163 No Hit GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG 2285 0.12598861085012814 No Hit GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC 2270 0.12516155213557587 No Hit GCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAA 2252 0.12416908167811316 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 2220 0.12240468975373499 No Hit GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCC 2177 0.12003378810535184 No Hit GCACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCT 2168 0.11953755287662049 RNA PCR Primer, Index 23 (96% over 25bp) GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA 2159 0.11904131764788911 No Hit ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGAT 2146 0.11832453342861049 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2141 0.11804884719042641 No Hit GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTC 2134 0.11766288645696868 No Hit TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA 2094 0.11545739655149598 No Hit CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTAC 2090 0.11523684756094871 No Hit GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACA 2090 0.11523684756094871 No Hit GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGG 2072 0.114244377103486 No Hit ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC 2071 0.11418923985584918 No Hit GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCA 2052 0.11314163215074965 No Hit GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATCACCTTAC 2002 0.11038476976890878 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 1999 0.1102193580259983 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 1992 0.1098333972925406 No Hit GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA 1890 0.1042093980335852 No Hit GTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTACACGGTGGA 1890 0.1042093980335852 No Hit GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGA 1870 0.10310665308084885 No Hit TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGC 1850 0.1020039081281125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 2.756862381840878E-4 0.0 2 0.0 0.0 0.0 2.756862381840878E-4 0.0 3 0.0 0.0 0.0 2.756862381840878E-4 0.0 4 0.0 0.0 0.0 2.756862381840878E-4 0.0 5 0.0 0.0 0.0 3.8596073345772297E-4 0.0 6 0.0 0.0 0.0 3.8596073345772297E-4 0.0 7 5.5137247636817564E-5 0.0 0.0 5.513724763681757E-4 0.0 8 5.5137247636817564E-5 0.0 0.0 5.513724763681757E-4 0.0 9 5.5137247636817564E-5 0.0 0.0 7.167842192786284E-4 0.0 10-11 5.5137247636817564E-5 0.0 0.0 0.0018195291720149798 0.0 12-13 5.5137247636817564E-5 0.0 0.0 0.003997450453669274 0.0 14-15 5.5137247636817564E-5 0.0 0.0 0.004659097425311084 0.0 16-17 5.5137247636817564E-5 0.0 0.0 0.008132744026430591 0.0 18-19 5.5137247636817564E-5 0.0 0.0 0.013674037413930758 0.0 20-21 5.5137247636817564E-5 0.0 0.0 0.02745834932313515 0.0 22-23 5.5137247636817564E-5 0.0 0.0 0.06845289294110901 0.0 24-25 5.5137247636817564E-5 0.0 0.0 0.16135915520914662 0.0 26-27 5.5137247636817564E-5 0.0 0.0 0.3020142739306682 0.0 28-29 5.5137247636817564E-5 0.0 0.0 0.5309165574949163 0.0 30-31 5.5137247636817564E-5 0.0 0.0 0.8769579236635834 0.0 32-33 5.5137247636817564E-5 0.0 0.0 1.4330722033285253 0.0 34-35 5.5137247636817564E-5 0.0 0.0 1.9505628410238764 0.0 36-37 5.5137247636817564E-5 0.0 0.0 2.5910095409493312 0.0 38-39 5.5137247636817564E-5 0.0 0.0 3.334783442946182 0.0 40-41 5.5137247636817564E-5 0.0 0.0 4.369874992832158 0.0 42-43 5.5137247636817564E-5 0.0 0.0 5.2467502106242865 0.0 44-45 5.5137247636817564E-5 0.0 0.0 6.05682665290441 0.0 46-47 5.5137247636817564E-5 0.0 0.0 6.832690433025888 0.0 48-49 5.5137247636817564E-5 0.0 0.0 7.513938696202588 0.0 50-51 5.5137247636817564E-5 0.0 0.0 8.174593197386935 0.0 52-53 1.1027449527363513E-4 5.5137247636817564E-5 0.0 8.933336862117184 0.0 54-55 1.1027449527363513E-4 1.1027449527363513E-4 0.0 9.62379304564923 0.0 56-57 1.1027449527363513E-4 1.1027449527363513E-4 0.0 10.316234170096203 0.0 58-59 1.1027449527363513E-4 1.1027449527363513E-4 0.0 11.036712584966498 0.0 60-61 1.1027449527363513E-4 1.1027449527363513E-4 0.0 11.684189283965647 0.0 62-63 1.1027449527363513E-4 1.1027449527363513E-4 0.0 12.314876691059386 0.0 64-65 1.1027449527363513E-4 1.1027449527363513E-4 0.0 12.960726841253248 0.0 66-67 1.1027449527363513E-4 1.1027449527363513E-4 0.0 13.589897974036973 0.0 68-69 1.1027449527363513E-4 1.1027449527363513E-4 0.0 14.23392859505882 0.0 70-71 1.1027449527363513E-4 1.1027449527363513E-4 0.0 14.946605089388505 0.0 72-73 1.1027449527363513E-4 1.1027449527363513E-4 0.0 15.630637783570865 0.0 74-75 1.1027449527363513E-4 1.1027449527363513E-4 0.0 16.26863087597648 0.0 76-77 1.1027449527363513E-4 1.1027449527363513E-4 0.0 16.943896747784585 0.0 78-79 1.1027449527363513E-4 1.1027449527363513E-4 0.0 17.581173055970922 0.0 80-81 1.1027449527363513E-4 1.1027449527363513E-4 0.0 18.351247425090534 0.0 82-83 1.1027449527363513E-4 1.1027449527363513E-4 0.0 19.49846056804598 0.0 84-85 1.1027449527363513E-4 1.1027449527363513E-4 0.0 20.137997503385428 0.0 86-87 1.1027449527363513E-4 1.1027449527363513E-4 0.0 20.769208714331715 0.0 88-89 1.1027449527363513E-4 1.1027449527363513E-4 0.0 21.359976754136397 0.0 90-91 1.1027449527363513E-4 1.1027449527363513E-4 0.0 22.025676313479515 0.0 92-93 1.1027449527363513E-4 1.1027449527363513E-4 0.0 22.56684839903488 0.0 94-95 1.1027449527363513E-4 1.1027449527363513E-4 0.0 23.110060562752807 0.0 96-97 1.378431190920439E-4 1.1027449527363513E-4 0.0 23.674721115801454 0.0 98-99 1.654117429104527E-4 1.1027449527363513E-4 0.0 24.251318882963474 0.0 100-101 1.654117429104527E-4 1.1027449527363513E-4 0.0 24.82259590572854 0.0 102-103 1.654117429104527E-4 1.1027449527363513E-4 0.0 25.39414861473179 0.0 104-105 1.654117429104527E-4 1.1027449527363513E-4 0.0 25.942240424865574 0.0 106-107 1.654117429104527E-4 1.1027449527363513E-4 0.0 26.446608397623365 5.5137247636817564E-5 108-109 1.654117429104527E-4 1.1027449527363513E-4 0.0 27.0266798113865 5.5137247636817564E-5 110-111 1.9298036672886148E-4 1.1027449527363513E-4 0.0 27.593380442597713 5.5137247636817564E-5 112-113 2.2054899054727026E-4 1.1027449527363513E-4 0.0 28.158206407389272 5.5137247636817564E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATAAC 550 0.0 89.787186 1 CGCATAG 910 0.0 84.34265 1 ATAACGC 605 0.0 81.62022 3 GTACAAC 360 0.0 79.33004 1 AGTGTAC 755 0.0 78.80034 3 GCATAGT 1075 0.0 72.49997 2 TACAACG 395 0.0 72.296814 2 TCACGCA 330 0.0 70.311386 4 TAGGACC 375 0.0 69.80659 4 ATAGTAC 1135 0.0 68.66737 4 AGATTAC 1265 0.0 67.25437 2 CATAGTA 1190 0.0 65.49367 3 CAGTGTA 940 0.0 64.55759 2 GTGTACT 945 0.0 64.21602 4 TAGTACT 1220 0.0 63.39552 5 GACCGTT 405 0.0 63.166737 7 TTAGGAC 420 0.0 62.327312 3 AGTTCTT 1030 0.0 61.227093 2 GCAAGTA 140 0.0 59.49425 2 CTTAGGA 420 0.0 59.49425 2 >>END_MODULE